Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy
Abstract
Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, IPIE, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, IPIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.
Cite
@article{arxiv.2009.07606,
title = {Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy},
author = {George Dwapanyin and Dirk Spangenberg and Alexander Heidt and Thomas Feurer and Gurthwin Bosman and Pieter Neethling and Erich Rohwer},
journal= {arXiv preprint arXiv:2009.07606},
year = {2020}
}
Comments
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