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Many important microscopy samples, such as liquid crystals, biological tissue, or starches, are birefringent in nature. They scatter light differently depending on the light polarization and molecular orientations. The complete…

Optics · Physics 2022-12-26 Herve Hugonnet , Moosung Lee , Seungwoo Shin , YongKeun Park

Diffusion MRI (dMRI) is a unique imaging technique for in vivo characterization of tissue microstructure and white matter pathways. However, its relatively long acquisition time implies greater motion artifacts when imaging, for example,…

Medical Physics · Physics 2020-02-26 Yoonmi Hong , Wei-Tang Chang , Geng Chen , Ye Wu , Weili Lin , Dinggang Shen , Pew-Thian Yap

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and…

We demonstrate that in situ coherent diffractive imaging (CDI), which harnesses the coherent interference between a strong and a weak beam illuminating a static and dynamic structure, can be a very dose-efficient imaging method. At low…

DNA and proteins are chiral: their three-dimensional structure cannot be superimposed with its mirror image. Circular dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case…

Biomolecules · Quantitative Biology 2020-09-09 Steven Daly , Frederic Rosu , Valerie Gabelica

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

In this paper, we propose a new model to segment cells in phase contrast microscopy images. Cell images collected from the similar scenario share a similar background. Inspired by this, we separate cells from the background in images by…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Lin Zhang

We demonstrate speckle noise reduction in an in-line holographic imaging system using a Zwitterion-doped liquid crystal dynamic scatterer (LCDS) cell diffuser. Integrated into a minimally modified bright-field microscope, the LCDS actively…

Segmentation of nuclei regions from histological images is an important task for automated computer-aided analysis of histological images, particularly in the presence of impermissible color variation in the color appearance of stained…

Image and Video Processing · Electrical Eng. & Systems 2025-06-10 Suman Mahapatra , Pradipta Maji

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

The development of compact indirect microwave holographic set-up by the implementation of low cost, specifically designed directive antennae as transmitter and receiver is proposed. Microwave holograms are recorded by 2D scanning over a…

Image and Video Processing · Electrical Eng. & Systems 2020-02-26 Vineeta Kumari , Aijaz Ahmed , Gyanendra Sheoran , Tirupathiraju Kanumuri , Chandra shakher

Segmentation is a key stage in dermoscopic image processing, where the accuracy of the border line that defines skin lesions is of utmost importance for subsequent algorithms (e.g., classification) and computer-aided early diagnosis of…

Computer Vision and Pattern Recognition · Computer Science 2019-02-21 Pedro M. M. Pereira , Rui Fonseca-Pinto , Rui Pedro Paiva , Luis M. N. Tavora , Pedro A. A. Assuncao , Sergio M. M. de Faria

Multiple sclerosis is an inflammatory autoimmune demyelinating disease that is characterized by lesions in the central nervous system. Typically, magnetic resonance imaging (MRI) is used for tracking disease progression. Automatic image…

Image and Video Processing · Electrical Eng. & Systems 2020-08-06 Nils Gessert , Julia Krüger , Roland Opfer , Ann-Christin Ostwaldt , Praveena Manogaran , Hagen H. Kitzler , Sven Schippling , Alexander Schlaefer

While analysing rare blood cell aggregates remains challenging in automated haematology, they could markedly advance label-free functional diagnostics. Conventional flow cytometers efficiently perform cell counting with leukocyte…

Automated in-vitro cell detection and counting have been a key theme for artificial and intelligent biological analysis such as biopsy, drug analysis and decease diagnosis. Along with the rapid development of microfluidics and lab-on-chip…

Computer Vision and Pattern Recognition · Computer Science 2020-01-08 Tiancheng Xia , Richard Jiang , YongQing Fu , Nanlin Jin

Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…

The inversion of linear systems is a fundamental step in many inverse problems. Computational challenges exist when trying to invert large linear systems, where limited computing resources mean that only part of the system can be kept in…

Distributed, Parallel, and Cluster Computing · Computer Science 2017-09-05 Yushan Gao , Thomas Blumensath

Infrared heterodyne interferometry has been proposed as a practical alternative for recombining a large number of telescopes over kilometric baselines in the mid-infrared. However, the current limited correlation capacities impose strong…

Instrumentation and Methods for Astrophysics · Physics 2020-07-08 Guillaume Bourdarot , Hugues Guillet de Chatellus , Jean-Philippe Berger

Limited-angle tomography of strongly scattering quasi-transparent objects is a challenging, highly ill-posed problem with practical implications in medical and biological imaging, manufacturing, automation, and environmental and food…

Image and Video Processing · Electrical Eng. & Systems 2024-08-15 Iksung Kang , Alexandre Goy , George Barbastathis