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The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…
We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…
Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…
Integrating optical diffraction tomography with imaging flow cytometry enables label-free quantifications of the three-dimensional (3D) morphology and hemoglobin content of red blood cells (RBCs) in their natural form. Self-rotation of RBCs…
Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…
Multispectral imaging (MSI) can provide information about tissue oxygenation, perfusion and potentially function during surgery. In this paper we present a novel, near real-time technique for intrinsic measurements of total haemoglobin…
We propose and demonstrate a holographic imaging scheme exploiting random illuminations for recording hologram and then applying numerical reconstruction and twin removal. We use an in-line holographic geometry to record the hologram in…
Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…
Holography has always held special appeal, for it is able to record and display spatial information in three dimensions. Here, we show how to augment the capabilities of digital holography by using a large number of narrow laser lines at…
Recently developed methods for rapid continuous volumetric two-photon microscopy facilitate the observation of neuronal activity in hundreds of individual neurons and changes in blood flow in adjacent blood vessels across a large volume of…
Laser Doppler holography (LDH) is a full-field imaging technique that was recently used in the human eye to reveal blood flow contrasts in the retinal and choroidal vasculature non-invasively, and with high temporal resolution. We here…
Digital pathology has recently been revolutionized by advancements in artificial intelligence, deep learning, and high-performance computing. With its advanced tools, digital pathology can help improve and speed up the diagnostic process,…
We investigate the problem of 3D complex field reconstruction corresponding to unstained red blood cells (RBCs) with a single defocused off-axis digital hologram. We employ recently introduced mean gradient descent (MGD) optimization…
Dichroic tomography is a 3D imaging technique in which the polarization of the incident beam is used to induce contrast due to the magnetization or orientation of a sample. The aim is to reconstruct not only the optical density but the…
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…
Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show…
Modeling of the red blood cell (RBC) shape is an integral part of the experimental and computer simulation investigations of light scattering by these cells for fundamental studies as well as diagnostic applications in techniques like…
We show, via three-dimensional immersed-boundary-finite-element-lattice-Boltzmann simulations, that deformability-based red blood cell (RBC) separation in deterministic lateral displacement (DLD) devices is possible. This is due to the…
Digital holography (DH) has been widely used for imaging and characterization of micro and nanostructures in materials science and biology and has the potential to provide high-resolution, non-destructive measurements of fluid surfaces as…