English
Related papers

Related papers: Modeling Fluorescence Correlation Spectroscopy thr…

200 papers

The wavelength calibration of spectrographs is an essential but challenging task in many disciplines. Calibration is traditionally accomplished by imaging the spectrum of a light source containing features that are known to appear at…

Instrumentation and Methods for Astrophysics · Physics 2018-04-04 Neil Thomas

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

Laser dynamics underpin a broad range of modern photonic technologies and continue to reveal rich nonlinear behaviors. However, existing spectroscopic tools, most notably time-stretched dispersive Fourier transform spectroscopy (TS-DFT),…

Using frequency-chirped radiation pressure slowing, we precisely control the velocity of a pulsed CaF molecular beam down to a few m/s, compressing its velocity spread by a factor of 10 while retaining high intensity: at a velocity of…

Atomic Physics · Physics 2017-04-05 S. Truppe , H. J. Williams , N. J. Fitch , M. Hambach , T. E. Wall , E. A. Hinds , B. E. Sauer , M. R. Tarbutt

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

Mesoscale and Nanoscale Physics · Physics 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

Diffraction tomography is a widely used inverse scattering technique for quantitative imaging of weakly scattering media. In its conventional formulation, diffraction tomography assumes monochromatic plane wave illumination. This…

Numerical Analysis · Mathematics 2026-03-11 Peter Elbau , Noemi Naujoks , Otmar Scherzer

In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and…

Optics · Physics 2007-05-23 Laurent Cognet , Françoise Coussen , Daniel Choquet , Brahim Lounis

Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…

Quantitative Methods · Quantitative Biology 2020-06-24 Martin Oheim , Adi Salomon , Maia Brunstein

We propose an approach to sensitively probe the chirality of molecules by measuring their coherent optical absorption spectra. It is shown that quantum dynamics of the cyclic three-level chiral molecules driven by appropriately-designed…

Quantum Physics · Physics 2015-05-28 W. Z. Jia , L. F. Wei

Microfading Spectrometry (MFS) is a method for assessing light sensitivity color (spectral) variations of cultural heritage objects. Each measured point on the surface gives rise to a time-series of stochastic observations that represents…

A cross-correlation technique of lensing tomography is presented to measure the evolution of dark energy in the universe. The variation of the weak lensing shear with redshift around massive foreground objects like bright galaxies and…

Astrophysics · Physics 2008-11-26 Bhuvnesh Jain , Andy Taylor

In numerous industrial processes involving fluids, viscosity is a determinant factor for reaction rates, flows, drying, mixing, etc. Its importance is even more determinant for phenomena observed are at the micro- and nano- scales as in…

We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…

Optics · Physics 2025-03-11 Yiyang Chen , Yuanxin Qiu , Matthew D. Lew

Despite advances in low-light level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of sub-diffraction limited imaging utilizing quantum…

Optics · Physics 2017-03-14 Yonatan Israel , Ron Tenne , Dan Oron , Yaron Silberberg

Diffuse correlation spectroscopy (DCS) is a noninvasive optical technique that probes microvascular blood flow in deep tissues. Here, we present and validate a new on-chip hardware correlator for high-speed DCS measurements. The correlator…

In this paper, we propose a theoretical framework for cooperative abnormality detection and localization systems by exploiting molecular communication setup. The system consists of mobile sensors in a fluidic medium, which are injected into…

Signal Processing · Electrical Eng. & Systems 2021-05-18 Ladan Khaloopour , Mahtab Mirmohseni , Masoumeh Nasiri-Kenari

We present a method for calculating the dynamics of a bosonic mixture, the multi-component correlated basis function (CBF) method. For single components, CBF results for the excitation energies agree quite well with experimental results,…

Quantum Gases · Physics 2017-03-29 Michael Rader , Martin Hebenstreit , Robert E. Zillich

Fluorescence-detected Fourier transform (FT) spectroscopy is a technique in which the relative paths of an optical interferometer are controlled to excite a material sample, and the ensuing fluorescence is detected as a function of the…