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Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Femtosecond laser filament-induced plasma spectroscopy (FIPS) demonstrates great potentials in the remote sensing for identifying atmospheric pollutant molecules. Due to the widespread aerosols in atmosphere, the remote detection based on…

Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus…

Optics · Physics 2022-02-01 Gargi Sharma , Kanwarpal Singh

Single molecule fluorescence tracking provides information at nm-scale and ms-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can…

Statistical Mechanics · Physics 2021-04-07 Horst-Holger Boltz , Alexei Sirbu , Nina Stelzer , Martin J. Lohse , Christof Schütte , Paolo Annibale

X-ray photon correlation spectroscopy was used to measure the diffusive dynamics of colloidal particles in a shear flow. The results presented here show how the intensity autocorrelation functions measure both the diffusive dynamics of the…

Soft Condensed Matter · Physics 2009-11-13 Sebastian Busch , Torben Haugaard Jensen , Yuriy Chushkin , Andrei Fluerasu

Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

In this article, we analyze different factors that affect the diffusion behavior of small tracer particles (as they are used e.g.in fluorescence correlation spectroscopy (FCS)) in the polymer network of a hydrogel and perform simulations of…

Soft Condensed Matter · Physics 2011-03-15 Fatemeh Tabatabaei , Olaf Lenz , Christian Holm

The excited state population of single solid-state emitters is subjected to energy fluctuations around the equilibrium driven by the bath and relaxation through the emission of phonons or photons. Simultaneous measurement of the associated…

Quantum Physics · Physics 2021-05-12 Hendrik Utzat , Moungi G. Bawendi

Strong coupling (SC) is a fundamental concept in physics that describes extreme interactions between light and matter. Recent experiments have demonstrated SC at the nanometer scale, where strongly confined polaritons, rather than photons,…

Vibrational spectroscopy is a powerful technique to characterize the near-equilibrium dynamics of molecules in the gas- and the condensed-phase. This contribution summarizes efforts from computer-based methods to gain insight into the…

Chemical Physics · Physics 2022-04-15 Markus Meuwly

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

The interplay between the oxidation state and the optical properties of molecules plays a key role for applications in displays, sensors or molecular-based memories. The fundamental mechanisms occurring at the level of a single-molecule…

Mesoscale and Nanoscale Physics · Physics 2018-08-15 Benjamin Doppagne , Michael C. Chong , Hervé Bulou , Alex Boeglin , Fabrice Scheurer , Guillaume Schull

Using photonic devices, we developed a new approach to traditional spectroscopy where the spectral cross-correlation with a template spectrum can be done entirely on-device. By creating photonic devices with a carefully designed, modulated…

Diffusing wave spectroscopy (DWS) can be employed as an optical rheology tool with numerous applications for studying the structure, dynamics and linear viscoelastic properties of complex fluids, foams, glasses and gels. To carry out DWS…

Soft Condensed Matter · Physics 2018-11-06 Chi Zhang , Mathias Reufer , Danila Gaudino , Frank Scheffold

Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on…

Quantitative Methods · Quantitative Biology 2015-05-13 Michael C. DeSantis , Shawn H. DeCenzo , Je-Luen Li , Y. M. Wang

The physical and chemical environment inside cells is of fundamental importance to all life but has traditionally been difficult to determine on a subcellular basis. Here we combine cutting-edge genomically integrated FRET biosensing to…

Cell Behavior · Quantitative Biology 2020-10-22 Jack W Shepherd , Sarah Lecinski , Jasmine Wragg , Sviatlana Shashkova , Chris MacDonald , Mark C Leake

X-ray photon correlation spectroscopy was used to probe the diffusive dynamics of colloidal particles in a shear flow. Combining X-ray techniques with microfluidics is an experimental strategy that reduces the risk of x-ray induced beam…

Soft Condensed Matter · Physics 2008-03-11 Andrei Fluerasu , Abdellatif Moussaid , Henri Gleyzolle , Peter Falus , Anders Madsen

Molecular Circular dichroism (CD) spectroscopy faces significant limitations due to the inherent weakness of chiroptical light-matter interactions. In this view, resonant optical antennas constitute a promising solution to this problem…

The present work deals with a curvature sensor that consists of two segments of asymmetric multicore fiber (MCF) fusion spliced with standard single mode fiber (SMF). The MCF comprises three strongly coupled cores; one of such cores is at…

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…