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For more than ten years now, many efforts have been done to identify and characterize nature of obstructed diffusion in model and cellular lipid membranes. Amongst all the techniques developed for this purpose, FCS, by means of…
Strong enhancement of molecular circular dichroism has the potential to enable efficient asymmetric photolysis, a method of chiral separation that has conventionally been impeded by insufficient yield and low enantiomeric excess. Here, we…
In the last two decades, Fresnel diffraction (FD) of a plane wave from phase steps has been systematically studied and applied for precise measurements of light wavelength, and height and refractive index of the step. In this study we…
In order to discern aggregation in solutions, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the…
We present a detection scheme for diffusing wave spectroscopy (DWS) based on a two cell geometry that allows efficient ensemble averaging. This is achieved by putting a fast rotating diffuser in the optical path between laser and sample. We…
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…
Robust velocity and position estimation is crucial for autonomous robot navigation. The optical flow based methods for autonomous navigation have been receiving increasing attentions in tandem with the development of micro unmanned aerial…
High contrast imaging and spectroscopy provide unique constraints for exoplanet formation models as well as for planetary atmosphere models. But this can be challenging because of the planet-to-star small angular separation and high flux…
An absolute measurement of the components of the shear rate tensor $\mathcal{S}$ in a fluid can be found by measuring the photon correlation function of light scattered from particles in the fluid. Previous methods of measuring…
Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…
We propose a compact MEMS-based optical autocorrelator based on a micromachined Michelson interferometer in silicon and the two-photon absorption non-linearity in a photodetector. The miniaturized autocorrelator has a scanning range of 1.2…
Diffraction is a manifestation of light at edge due to its wavelike nature. The well-known diffraction phenomena are Fresnel and Fraunhofer, they find variety of applications individually. But the synergy of two phenomena is not studied and…
Light rays incident on a transparent object of uniform refractive index undergo deflections, which uniquely characterize the surface geometry of the object. Associated with each point on the surface is a deflection map (or spectrum) which…
Interferometric photon-correlation measurements, which correspond to the second-order intensity cross-correlations between the two output ports of an unbalanced Michelson interferometer, are sensitive to both amplitude and phase…
A method is described intended for distributed calibration of a probe microscope scanner consisting in a search for a net of local calibration coefficients (LCCs) in the process of automatic measurement of a standard surface, whereby each…
The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…
Intermediate Scattering Functions (ISF's) are measured for colloidal hard sphere systems using both Dynamic Light Scattering (DLS) and X-ray Photon Correlation Spectroscopy (XPCS). We compare the techniques, and discuss the advantages and…
Here, we discuss a collection of cutting-edge techniques and applications in use today by some of the leading experts in the field of correlative approaches in single-molecule biophysics. A key difference in emphasis, compared with…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…