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Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

Optics · Physics 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

Robust and accurate nuclei centroid detection is important for the understanding of biological structures in fluorescence microscopy images. Existing automated nuclei localization methods face three main challenges: (1) Most of object…

Image and Video Processing · Electrical Eng. & Systems 2021-11-05 Liming Wu , Shuo Han , Alain Chen , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Four-dimensional scanning transmission electron microscopy (4D-STEM) enables mapping of diffraction information with nanometer-scale spatial resolution, offering detailed insight into local structure, orientation, and strain. However, as…

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Instrumental aberrations strongly limit high-contrast imaging of exoplanets, especially when they produce quasistatic speckles in the science images. With the help of recent advances in deep learning, we have developed in previous works an…

Instrumentation and Methods for Astrophysics · Physics 2022-11-14 Maxime Quesnel , Gilles Orban de Xivry , Olivier Absil , Gilles Louppe

Accurate three-dimensional (3D) imaging requires an isotropic point spread function (PSF). However, the inherent missing aperture of a single objective lens results in an elongated, cigar-like PSF, which has rendered isotropic resolution in…

Light sheet fluorescence microscopy (LSM) enables high-resolution, three-dimensional (3D) imaging of biological specimens, providing rich volumetric data for studying cellular organization, pathology, and vascular networks. However, the…

Computer Vision and Pattern Recognition · Computer Science 2026-05-26 Adina Scheinfeld , Haotan Zhang , Shang Mu , Rudolf L. M. van Herten , Lucas Stoffl , Ali Erturk , Zhuhao Wu , Johannes C. Paetzold

Based on point spread function (PSF) engineering and astigmatism due to a pair of cylindrical lenses, a novel compressed imaging mechanism is proposed to achieve single-shot incoherent 3D imaging. The speckle-like PSF of the imaging system…

Optics · Physics 2021-05-24 Qiong Gao , Weidong Qu , Ming Shao , Wei Liu , Xiangzheng Cheng

Large-scale astronomical surveys can capture numerous images of celestial objects, including galaxies and nebulae. Analysing and processing these images can reveal intricate internal structures of these objects, allowing researchers to…

Instrumentation and Methods for Astrophysics · Physics 2023-11-02 Peng Jia , Jiameng Lv , Runyu Ning , Yu Song , Nan Li , Kaifan Ji , Chenzhou Cui , Shanshan Li

Pansharpening is a crucial task in remote sensing, enabling the generation of high-resolution multispectral images by fusing low-resolution multispectral data with high-resolution panchromatic images. This paper provides a comprehensive…

Image and Video Processing · Electrical Eng. & Systems 2024-12-09 Mahek Kantharia , Neeraj Badal , Zankhana Shah

Point spread function (PSF) engineering has been pivotal in the remarkable progress made in high-resolution imaging in the last decades. However, the diversity in PSF structures attainable through existing engineering methods is limited.…

Optics · Physics 2025-06-10 Md Sadman Sakib Rahman , Aydogan Ozcan

Scanning Kelvin probe microscopy (SKPM) is a powerful technique for investigating the electrostatic properties of material surfaces, enabling the imaging of variations in work function, topology, surface charge density, or combinations…

Applied Physics · Physics 2024-07-26 Isaac C. D. Lenton , Felix Pertl , Lubuna Shafeek , Scott R. Waitukaitis

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

How microorganisms respond to and interact with their environment can vary significantly from individual to individual, which can have important microbiological and ecological implications. However, most microscopy techniques can only…

Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Sachin Shah , Sakshum Kulshrestha , Christopher A. Metzler

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…

Simulated images are essential in algorithm development and instrument testing for optical telescopes. During real observations, images obtained by optical telescopes are affected by spatially variable point spread functions (PSFs), a…

Instrumentation and Methods for Astrophysics · Physics 2025-02-17 Zeyu Bai , Peng Jia , Jiameng Lv , Xiang Zhang , Wennan Xiang , Lin Nie

Estimating the pose of a moving camera from monocular video is a challenging problem, especially due to the presence of moving objects in dynamic environments, where the performance of existing camera pose estimation methods are susceptible…

Computer Vision and Pattern Recognition · Computer Science 2022-07-20 Wang Zhao , Shaohui Liu , Hengkai Guo , Wenping Wang , Yong-Jin Liu

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Point management is critical for optimizing 3D Gaussian Splatting models, as point initiation (e.g., via structure from motion) is often distributionally inappropriate. Typically, Adaptive Density Control (ADC) algorithm is adopted,…

Computer Vision and Pattern Recognition · Computer Science 2025-04-22 Haosen Yang , Chenhao Zhang , Wenqing Wang , Marco Volino , Adrian Hilton , Li Zhang , Xiatian Zhu
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