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Solid-state nanopores are promising tools for single molecule detection of both DNA and proteins. In this study, we investigate the patterns of ionic current blockades as DNA translocates into or out of the geometric confinement of such…

Biological Physics · Physics 2017-04-05 Kaikai Chen , Nicholas A. W. Bell , Jinglin Kong , Yu Tian , Ulrich F. Keyser

We investigate the ionic current modulation in DNA nanopore translocation setups by numerically solving the electrokinetic mean-field equations for an idealized model. Specifically, we study the dependence of the ionic current on the…

Soft Condensed Matter · Physics 2021-10-13 Kai Szuttor , Patrick Kreissl , Christian Holm

Solid-state nanopores are single molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage and salt dependence of the frequency of…

Biological Physics · Physics 2016-03-23 Nicholas A. W. Bell , Murugappan Muthukumar , Ulrich F. Keyser

The passage of DNA through a nanopore can be effectively decomposed into two distinct phases, docking and actual translocation. In experiments each phase is characterized by a distinct current signature which allows the discrimination of…

We investigate the salt-dependent current modulation of bundled DNA nanostructures in a nanopore. To this end, we developed four simulation models for a 2x2 origami structure with increasing level of detail: from the mean-field level to an…

Soft Condensed Matter · Physics 2021-02-24 Kai Szuttor , Florian Weik , Jean-Noël Grad , Christian Holm

Double-stranded DNA translocates through sufficiently large nanopores either in a linear, single-file fashion or in a folded hairpin conformation when captured somewhere along its length. We show that the folding state of DNA can be…

Biological Physics · Physics 2018-08-15 Niklas Ermann , Nikita Hanikel , Vivian Wang , Kaikai Chen , Ulrich F. Keyser

Ionic currents accompanying DNA translocation strongly depend on molarity of the electrolyte solution and the shape and surface charge of the nanopore. By means of the Poisson-Nernst-Planck equations it is shown how conductance is modulated…

Soft Condensed Matter · Physics 2015-07-16 Valerio Mazzone , Simone Melchionna , Umberto Marini Bettolo Marconi

The structure of DNA double helix is stabilized by metal counterions condensed to a diffuse layer around the macromolecule. The dynamics of counterions in real conditions is governed by the electric fields from DNA and other biological…

Biological Physics · Physics 2020-10-27 O. O. Zdorevskyi , S. M. Perepelytsya

Molecular dynamics simulations are used to study the equilibrium distribution of monovalent ions in a nanopore connecting two water reservoirs separated by a membrane, both for the empty pore and that with a single stranded DNA molecule…

Soft Condensed Matter · Physics 2009-11-10 Yitzhak Rabin , Motohiko Tanaka

Nanopore based sequencing has demonstrated significant potential for the development of fast, accurate, and cost-efficient fingerprinting techniques for next generation molecular detection and sequencing. We propose a specific multi-layered…

Mesoscale and Nanoscale Physics · Physics 2014-05-15 Towfiq Ahmed , Jason T. Haraldsen , John J. Rehr , Massimiliano Di Ventra , Ivan K. Schuller , Alexander V. Balatsky

A solid-state nanopore can electrophoretically capture a DNA molecule and pull it through in a folded configuration. The resulting ionic current signal indicates where along its length the DNA was captured. A statistical study using an 8 nm…

Biological Physics · Physics 2015-06-11 Mirna Mihovilovic , Nick Hagerty , Derek Stein

We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the…

The threading of a polymer chain through a small pore is a classic problem in polymer dynamics and underlies nanopore sensing technology. However important experimental aspects of the polymer motion in a solid-state nanopore, such as an…

Biological Physics · Physics 2016-07-18 Nicholas A. W. Bell , Ulrich F. Keyser

Novel approaches to DNA sequencing and detection require the measurement of electrical currents between metal probes immersed in ionic solution. Here, we experimentally demonstrate that these systems maintain large background currents with…

Graphene nanopore based sensor devices are exhibiting the great potential for the detection of DNA. To understand the fundamental aspects of DNA translocating through a graphene nanopore, in this work, molecular dynamics (MD) simulations…

Biological Physics · Physics 2012-11-30 Wenping Lv , Maodu Chen , Renan Wu

The requirement to boost the resolution of nanopore-based biosequencing devices necessitates the integration of novel biosensing techniques with reduced sensitivity to background noise. In this article, we probe the signatures of…

Soft Condensed Matter · Physics 2023-05-01 Sahin Buyukdagli

Recent experiments of translocation of double stranded DNA through nanopores [M. Wanunu \textit{et al.} Nature Nanotech. {\bf 5}, 160 (2010)] reveal that the DNA capture rate can be significantly influenced by a salt gradient across the…

Soft Condensed Matter · Physics 2015-03-17 Marius M. Hatlo , Debabrata Panja , René van Roij

Extracting the sequence information of DNA from the blocked ionic current is the crucial step of the ionic current-based nanopore sequencing approaches. The thinnest graphene nanopore, which contained only one layer of carbon atoms,…

Biological Physics · Physics 2014-01-29 Wenping Lv , Ren'an Wu

We predict streaming current inversion with multivalent counterions in hydrodynamically driven polymer translocation events from a correlation-corrected charge transport theory including charge fluctuations around mean-field electrostatics.…

Soft Condensed Matter · Physics 2015-03-05 Sahin Buyukdagli , Ralf Blossey , T. Ala-Nissila

We report translocation experiments on double-strand DNA through a silicon oxide nanopore. Samples containing DNA fragments with seven different lengths between 2000 to 96000 basepairs have been electrophoretically driven through a 10 nm…

Biomolecules · Quantitative Biology 2015-06-26 Arnold J. Storm , Cornelis Storm , Jianghua Chen , Henny Zandbergen , Jean-Francois Joanny , Cees Dekker
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