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Scanning Electron Microscopy (SEM) is indispensable in modern materials science, enabling high-resolution imaging across a wide range of structural, chemical, and functional investigations. However, SEM imaging remains constrained by…

We target the problem of estimating the center of mass of noisy 2-D images. We assume that the noise dominates the image, and thus many standard approaches are vulnerable to estimation errors. Our approach uses a surrogate function to the…

Image and Video Processing · Electrical Eng. & Systems 2021-03-04 Ayelet Heimowitz , Nir Sharon , Amit Singer

Direct detector device (DDD) cameras have revolutionized single particle electron cryomicroscopy (cryo-EM). In addition to an improved camera detective quantum efficiency, acquisition of DDD movies allows for correction of movement of the…

Quantitative Methods · Quantitative Biology 2015-11-23 John L. Rubinstein , Marcus A. Brubaker

Diffusion models have demonstrated significant potential in producing high-quality images in medical image translation to aid disease diagnosis, localization, and treatment. Nevertheless, current diffusion models have limited success in…

Image and Video Processing · Electrical Eng. & Systems 2024-11-26 Yunxiang Li , Hua-Chieh Shao , Xiaoxue Qian , You Zhang

In this paper, we present a novel Bayesian approach for estimating spectral and range profiles from single-photon Lidar waveforms associated with single surfaces in the photon-limited regime. In contrast to classical multispectral Lidar…

Image and Video Processing · Electrical Eng. & Systems 2019-12-13 Quentin Legros , Sylvain Meignen , Stephen McLaughlin , Yoann Altmann

Single-particle imaging with X-ray free-electron lasers depends crucially on algorithms that merge large numbers of weak diffraction patterns despite missing measurements of parameters such as particle orientations. The…

Computational Physics · Physics 2021-08-24 B. R. Mobley , K. E. Schmidt , J. P. Chen , R. A. Kirian

Traditional image acquisition for cryo focused ion-beam scanning electron microscopy tomography often sees thousands of images being captured over a period of many hours, with immense data sets being produced. When imaging beam sensitive…

Dielectric tensor tomography is an imaging technique for mapping three-dimensional distributions of dielectric properties in transparent materials. This work introduces an enhanced illumination strategy employing a micro-electromechanical…

Differentiating signals from the background in micrographs is a critical initial step for cryogenic electron microscopy (cryo-EM), yet it remains laborious due to low signal-to-noise ratio (SNR), the presence of contaminants and densely…

Image and Video Processing · Electrical Eng. & Systems 2025-09-30 Szu-Chi Chung , Po-Cheng Chou

The appearance of direct electron detectors marked a new era for electron diffraction. Their high sensitivity and low noise opens the possibility to extend electron diffraction from transmission electron microscopes (TEM) to lower energies…

Applied Physics · Physics 2025-02-18 Nikita Denisov , Andrey Orekhov , Johan Verbeeck

Magnetic Resonance Imaging (MRI) is a powerful imaging technique widely used for visualizing structures within the human body and in other fields such as plant sciences. However, there is a demand to develop fast 3D-MRI reconstruction…

Image and Video Processing · Electrical Eng. & Systems 2025-12-09 Arya Bangun , Zhuo Cao , Alessio Quercia , Hanno Scharr , Elisabeth Pfaehler

Cryo Focused Ion-Beam Scanning Electron Microscopy (cryo FIB-SEM) enables three-dimensional and nanoscale imaging of biological specimens via a slice and view mechanism. The FIB-SEM experiments are, however, limited by a slow (typically,…

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the…

Computational Physics · Physics 2018-03-20 Amit Singer

Latest diffusion-based methods for many image restoration tasks outperform traditional models, but they encounter the long-time inference problem. To tackle it, this paper proposes a Wavelet-Based Diffusion Model (WaveDM). WaveDM learns the…

Computer Vision and Pattern Recognition · Computer Science 2024-01-26 Yi Huang , Jiancheng Huang , Jianzhuang Liu , Mingfu Yan , Yu Dong , Jiaxi Lv , Chaoqi Chen , Shifeng Chen

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

High-resolution transmission electron microscopy (HRTEM) is an important method for imaging beam sensitive materials often under cryo conditions. Electron ptychography in the scanning transmission electron microscope (STEM) has been shown…

Materials Science · Physics 2025-09-19 Felix Bennemann , Angus I. Kirkland , David A. Muller , Peter Nellist

Addressing the need for efficient and integrated multiscale crystallographic and defect analyses of advanced materials, this paper presents the implementation of a new multi-configuration detection system, integrating a single…

Instrumentation and Detectors · Physics 2026-05-19 Nohayla El-Khairaoui , Julien Guyon , Nathalie Gey , Luc Morhain , Nabila Maloufi

A common task in single particle electron cryomicroscopy (cryo-EM) is the rigid alignment of images and/or volumes. In the context of images, a rigid alignment involves estimating the inner-product between one image of $N\times N$ pixels…

Numerical Analysis · Mathematics 2022-12-07 Aaditya V. Rangan

Cryo-electron microscopy (cryo-EM) has emerged as a powerful technique for resolving the three-dimensional structures of macromolecules. A key challenge in cryo-EM is characterizing continuous heterogeneity, where molecules adopt a…

Machine Learning · Statistics 2026-02-20 Roey Yadgar , Roy R. Lederman , Yoel Shkolnisky

Cryo-electron tomography (cryo-ET) is an imaging technique that allows three-dimensional visualization of macro-molecular assemblies under near-native conditions. Cryo-ET comes with a number of challenges, mainly low signal-to-noise and…