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Positron Emission Tomography (PET) is an important clinical imaging tool but inevitably introduces radiation hazards to patients and healthcare providers. Reducing the tracer injection dose and eliminating the CT acquisition for attenuation…

Computer Vision and Pattern Recognition · Computer Science 2024-06-18 Tianqi Chen , Jun Hou , Yinchi Zhou , Huidong Xie , Xiongchao Chen , Qiong Liu , Xueqi Guo , Menghua Xia , James S. Duncan , Chi Liu , Bo Zhou

Wavelet functions allow the sparse and efficient representation of a signal at different scales. Recently the application of wavelets to the denoising of maps of cosmic microwave background (CMB) fluctuations has been proposed. The…

Astrophysics · Physics 2009-11-07 Klaus Maisinger , M. P. Hobson , A. N. Lasenby

It has been a general trend to develop low-voltage electron microscopes due to their high imaging contrast of the sample and low radiation damage. Atom-resolved transmission electron microscopes with voltages as low as 15-40 kV have been…

Instrumentation and Detectors · Physics 2015-12-29 Wei-Tse Chang , Chun-Yueh Lin , Wei-Hao Hsu , Mu-Tung Chang , Yi-Sheng Chen , En-Te Hwu , Ing-Shouh Hwang

Cellular Electron Cryo-Tomography (CECT) is a powerful 3D imaging tool for studying the native structure and organization of macromolecules inside single cells. For systematic recognition and recovery of macromolecular structures captured…

Computer Vision and Pattern Recognition · Computer Science 2018-07-04 Chang Liu , Xiangrui Zeng , Kaiwen Wang , Qiang Guo , Min Xu

Laser flash melting and revitrification experiments have recently improved the time resolution of cryo-electron microscopy (cryo-EM) to the microsecond timescale, making it fast enough to observe many of the protein motions that are…

Instrumentation and Detectors · Physics 2026-05-19 Wyatt A. Curtis , Constantin R. Krüger , Axel P. Tracol Gavard , Jakub Wenz , Marcel Drabbels , Ulrich J. Lorenz

Understanding protein flexibility and its dynamic interactions with other molecules is essential for studying protein function. Although cryogenic electron microscopy(cryo-EM) provides an opportunity to observe macromolecular dynamics…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Bintao He , Yiran Cheng , Hongjia Li , Xiang Gao , Xin Gao , Fa Zhang , Renmin Han

Generating consistent multiple views for 3D reconstruction tasks is still a challenge to existing image-to-3D diffusion models. Generally, incorporating 3D representations into diffusion model decrease the model's speed as well as…

Computer Vision and Pattern Recognition · Computer Science 2024-06-14 Emmanuelle Bourigault , Pauline Bourigault

Single-Particle Reconstruction (SPR) in Cryo-Electron Microscopy (cryo-EM) is the task of estimating the 3D structure of a molecule from a set of noisy 2D projections, taken from unknown viewing directions. Many algorithms for SPR start…

Optimization and Control · Mathematics 2018-01-09 Eitan Levin , Tamir Bendory , Nicolas Boumal , Joe Kileel , Amit Singer

In this report, we applied expectation and maximization (EM) method described by Philips et al [1] to recover two-dimensional (2D) structure from multiple sparse signal images in random orientation. The detailed derivation of EM algorithm…

Data Analysis, Statistics and Probability · Physics 2017-01-12 Yun Zhao

Cryo-electron tomography (cryo-ET) has emerged as a powerful tool for studying the structural heterogeneity of proteins and their complexes, offering insights into macromolecular dynamics directly within cells. Driven by recent…

Biomolecules · Quantitative Biology 2025-07-01 Jackson Carrion , Joseph H. Davis

Monocular depth estimation (MDE) aims to infer per-pixel depth from a single RGB image. While diffusion models have advanced MDE with impressive generalization, they often exhibit limitations in accurately reconstructing far-range regions.…

Computer Vision and Pattern Recognition · Computer Science 2025-11-18 Mingxia Zhan , Li Zhang , Yingjie Wang , Xiaomeng Chu , Beibei Wang , Yanyong Zhang

Cryo-EM is a transformational paradigm in molecular biology where computational methods are used to infer 3D molecular structure at atomic resolution from extremely noisy 2D electron microscope images. At the forefront of research is how to…

Quantitative Methods · Quantitative Biology 2025-06-12 Shayan Shekarforoush , David B. Lindell , Marcus A. Brubaker , David J. Fleet

Limited-angle electron tomography aims to reconstruct 3D shapes from 2D projections of Transmission Electron Microscopy (TEM) within a restricted range and number of tilting angles, but it suffers from the missing-wedge problem that causes…

Computer Vision and Pattern Recognition · Computer Science 2026-05-05 Zhantao Deng , Mériem Er-Rafik , Anna Sushko , Cécile Hébert , Pascal Fua

Optical endomicroscopy (OEM) is an emerging technology platform with preclinical and clinical imaging applications. Pulmonary OEM via fibre bundles has the potential to provide in vivo, in situ molecular signatures of disease such as…

Computer Vision and Pattern Recognition · Computer Science 2018-08-29 Ahmed Karam Eldaly , Yoann Altmann , Antonios Perperidis , Nikola Krstajic , Tushar Choudhary , Kevin Dhaliwal , Stephen McLaughlin

Advances on cryo-electron imaging technologies have led to a rapidly increasing number of density maps. Alignment and comparison of density maps play a crucial role in interpreting structural information, such as conformational…

Computer Vision and Pattern Recognition · Computer Science 2023-09-19 Bintao He , Fa Zhang , Chenjie Feng , Jianyi Yang , Xin Gao , Renmin Han

The limited dynamic range of the detector can impede coherent diffractive imaging (CDI) schemes from achieving diffraction-limited resolution. To overcome this limitation, a straightforward approach is to utilize high dynamic range (HDR)…

Image and Video Processing · Electrical Eng. & Systems 2024-06-11 Shantanu Kodgirwar , Lars Loetgering , Chang Liu , Aleena Joseph , Leona Licht , Daniel S. Penagos Molina , Wilhelm Eschen , Jan Rothhardt , Michael Habeck

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Advances in imagery at atomic and near-atomic resolution, such as cryogenic electron microscopy (cryo-EM), have led to an influx of high resolution images of proteins and other macromolecular structures to data banks worldwide. Producing a…

Quantitative Methods · Quantitative Biology 2021-04-06 Nathan Ranno , Dong Si

Cryo-electron microscopy (cryo-EM) is an experimental technique for protein structure determination that images an ensemble of macromolecules in near-physiological contexts. While recent advances enable the reconstruction of dynamic…

Electron microscopy has shown to be a very powerful tool to map the chemical nature of samples at various scales down to atomic resolution. However, many samples can not be analyzed with an acceptable signal-to-noise ratio because of the…

Image and Video Processing · Electrical Eng. & Systems 2018-02-28 Étienne Monier , Thomas Oberlin , Nathalie Brun , Marcel Tencé , Marta de Frutos , Nicolas Dobigeon