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Recent advances in imaging sensors and digital light projection technology have facilitated a rapid progress in 3D optical sensing, enabling 3D surfaces of complex-shaped objects to be captured with improved resolution and accuracy.…

Instrumentation and Detectors · Physics 2017-11-22 Chao Zuo , Tianyang Tao , Shijie Feng , Lei Huang , Anand Asundi , Qian Chen

Optical coherence tomography (OCT) is capable of non-destructively obtaining cross-sectional information of samples with micrometer spatial resolution, which plays an important role in ophthalmology and endovascular medicine. Measuring OCT…

Medical Physics · Physics 2023-11-30 Yang Jianlong , Zhang Haoran , Liu Chang , Gu Chengfu

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

Background: Accurate segmentation of diffuse large B-cell lymphoma (DLBCL) lesions is challenging due to their complex patterns in medical imaging. Objective: This study aims to develop a precise segmentation method for DLBCL using…

Image and Video Processing · Electrical Eng. & Systems 2024-09-10 Huan Huang , Liheng Qiu , Shenmiao Yang , Longxi Li , Jiaofen Nan , Yanting Li , Chuang Han , Fubao Zhu , Chen Zhao , Weihua Zhou

Coherent Diffractive Imaging is a lensless technique that allows imaging of matter at a spatial resolution not limited by lens aberrations. This technique exploits the measured diffraction pattern of a coherent beam scattered by periodic…

Computational Physics · Physics 2025-08-27 Alessandro Colombo , Davide Emilio Galli , Liberato De Caro , Francesco Scattarella , Elvio Carlino

Complex field imaging, which captures both the amplitude and phase information of input optical fields or objects, can offer rich structural insights into samples, such as their absorption and refractive index distributions. However,…

Optics · Physics 2024-05-30 Jingxi Li , Yuhang Li , Tianyi Gan , Che-Yung Shen , Mona Jarrahi , Aydogan Ozcan

Scanning ion conductance microscopy (SICM) can image the surface topography of specimens in ionic solutions without mechanical probe--sample contact. This unique capability is advantageous for imaging fragile biological samples but its…

Applied Physics · Physics 2019-12-25 Shinji Watanabe , Satoko Kitazawa , Linhao Sun , Noriyuki Kodera , Toshio Ando

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

We report a time-resolved single photon counting (TCSPC) imaging system based on a line-scanning architecture. The system benefits from the high fill-factor, active area, and large dimension of an advanced CMOS single photon avalanche diode…

Fast, volumetric imaging that integrates multiple imaging modalities is essential for probing dynamic, heterogeneous soft and biological matter. Here, we present the M3Scope, a simple yet versatile multiplane microscope that extends…

MFC is an open-source tool for solving multi-component, multi-phase, and bubbly compressible flows. It is capable of efficiently solving a wide range of flows, including droplet atomization, shock-bubble interaction, and gas bubble…

Computational Physics · Physics 2020-08-19 Spencer H. Bryngelson , Kevin Schmidmayer , Vedran Coralic , Jomela C. Meng , Kazuki Maeda , Tim Colonius

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Three-dimensional (3D) imaging of thin, extended specimens at nanometer resolution is critical for applications in biology, materials science, advanced synthesis, and manufacturing. One route to 3D imaging is tomography, which requires a…

Instrumentation and Detectors · Physics 2023-10-23 Deepan Balakrishnan , See Wee Chee , Zhaslan Baraissov , Michel Bosman , Utkur Mirsaidov , N. Duane Loh

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Multi-focus image fusion (MFIF) addresses the depth-of-field (DOF) limitations of optical lenses, where only objects within a specific range appear sharp. Although traditional and deep learning methods have advanced the field, challenges…

Computer Vision and Pattern Recognition · Computer Science 2025-10-23 Luca Piano , Peng Huanwen , Radu Ciprian Bilcu

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

Event cameras are novel bio-inspired sensors that offer advantages over traditional cameras (low latency, high dynamic range, low power, etc.). Optical flow estimation methods that work on packets of events trade off speed for accuracy,…

Computer Vision and Pattern Recognition · Computer Science 2024-03-05 Shintaro Shiba , Yoshimitsu Aoki , Guillermo Gallego

Microfluidic fuel cells (MFCs) are microfluidic electrochemical conversion devices that are used to power small pieces of electrical equipment. Their performance relies on the improvement of the mass transfer of the reactants at the…

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich