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The immunohistochemical (IHC) staining of the human epidermal growth factor receptor 2 (HER2) biomarker is widely practiced in breast tissue analysis, preclinical studies and diagnostic decisions, guiding cancer treatment and investigation…

Fluorescence-detected Fourier transform (FT) spectroscopy is a technique in which the relative paths of an optical interferometer are controlled to excite a material sample, and the ensuing fluorescence is detected as a function of the…

Transient absorption (TA) spectroscopy has proved fundamental to our understanding of energy and charge transfer in biological systems, allowing measurements of photoactive proteins on sub-picosecond timescales. Recently, ultrafast TA…

Dynabeads are superparamagnetic particles used for immunomagnetic purification of cells and biomolecules. Post-capture, however, target identification relies on tedious culturing, fluorescence staining and/or target amplification. Raman…

Biological Physics · Physics 2023-07-25 Jongwan Lee , Marissa McDonald , Nikiwe Mhlanga , Jeon Woong Kang , Rohit Karnik , Loza F. Tadesse

Catheter ablation has limited therapeutic efficacy against non-paroxysmal atrial fibrillation (AF), and electrophysiological studies using mapping catheters have been applied to evaluate the AF substrate. However, many of these approaches…

Signal Processing · Electrical Eng. & Systems 2024-08-08 Hiroshi Seno , Toshiya Kojima , Masatoshi Yamazaki , Ichiro Sakuma , Katsuhito Fujiu , Naoki Tomii

Multiplex immunofluorescence and immunohistochemistry benefit patients by allowing cancer pathologists to identify several proteins expressed on the surface of cells, enabling cell classification, better understanding of the tumour…

Computer Vision and Pattern Recognition · Computer Science 2021-07-19 Jessica Cooper , In Hwa Um , Ognjen Arandjelović , David J Harrison

We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a…

Optics · Physics 2015-05-13 Kevin McHale , Hideo Mabuchi

The Advanced Superconducting Test Accelerator (ASTA) currently under commissioning at Fermilab will enable a broad range of beam-based experiments to study fundamental limitations to beam intensity and to develop transformative approaches…

Accelerator Physics · Physics 2014-09-23 V. Shiltsev

While model architectures and training strategies have become more generic and flexible with respect to different data modalities over the past years, a persistent limitation lies in the assumption of fixed quantities and arrangements of…

Image and Video Processing · Electrical Eng. & Systems 2023-11-07 Lisa Weijler , Florian Kowarsch , Michael Reiter , Pedro Hermosilla , Margarita Maurer-Granofszky , Michael Dworzak

Antibodies are proteins produced by the immune system that recognize and bind to specific antigens, and their 3D structures are crucial for understanding their binding mechanism and designing therapeutic interventions. The specificity of…

Machine Learning · Computer Science 2024-10-23 Jiying Zhang , Zijing Liu , Shengyuan Bai , He Cao , Yu Li , Lei Zhang

Modern embedded Linux devices, such as routers, IP cameras, and IoT gateways, rely on complex software stacks where numerous daemons interact to provide services. Testing these devices is crucial from a security perspective since vendors…

Cryptography and Security · Computer Science 2025-09-23 Alessio Izzillo , Riccardo Lazzeretti , Emilio Coppa

This paper introduces an innovative two-core fiber (TCF) optic sensor employing a Mach-Zehnder interferometer (MZI) to monitor the optogenetic response of light-sensitive human dental pulp stem cells (hDPSCs). The in-fiber MZI, formed using…

We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…

Photoacoustic tomography (PAT) is intrinsically sensitive to blood oxygen saturation (sO2) in vivo. However, making accurate sO2 measurements without knowledge of tissue- and instrumentation-related correction factors is extremely…

Medical Physics · Physics 2019-12-17 Marcel Gehrung , Sarah Bohndiek , Joanna Brunker

FoF1-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for cellular processes. In Escherichia coli ATP synthesis is…

Biomolecules · Quantitative Biology 2015-05-27 Karin Seyfert , Takuya Oosaka , Hideyuki Yaginuma , Stefan Ernst , Hiroyuki Noji , Ryota Iino , Michael Boersch

Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with correct antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, we report a phenotypic platform that rapidly…

Fluorescence imaging and spectroscopy remain the most powerful tools for visualization with chemical and immunological specificity of labeled biomolecules, viruses, cellular organelles, and living cells in complex biological backgrounds.…

Angle-resolved photoemission spectroscopy (ARPES) is a powerful probe of electron correlations in two-dimensional layered materials. In this Letter we demonstrate that ARPES can be used to probe the onset of exciton condensation in…

Strongly Correlated Electrons · Physics 2013-02-25 Stefan Rist , A. A. Varlamov , A. H. MacDonald , Rosario Fazio , Marco Polini

Angle-resolved photoemission spectroscopy is the leading tool for studying the symmetry and structure of the order parameter in superconductors. The recent improvement of the technique made it possible to detect the superconducting energy…

The combination of single cells to form doublets is regarded as the rate-limiting step of flocculation and requires the presence of surface proteins in active form. The process of activation of the flocculation proteins of yeast cells is…

Biological Physics · Physics 2007-05-23 Silvia Stan , Florin Despa