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Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

A technique to measure microscopic rotational motion is presented. When a small fluorescent polystyrene microsphere is attached to a larger polystyrene microsphere, the larger sphere acts as a lens for the smaller microsphere and provides…

Soft Condensed Matter · Physics 2009-10-31 James P. Brody , Stephen R. Quake

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

Optics · Physics 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky

We study the diffraction of a particular class of beams, composed only by a combination of azimuthally invariant guided modes of an optical fiber. We demonstrate that such beams can be obtained by injecting a Gaussian beam in a small piece…

We present a superpixel method for full spatial phase and amplitude control of a light beam using a digital micromirror device (DMD) combined with a spatial filter. We combine square regions of nearby micromirrors into superpixels by low…

Optics · Physics 2015-06-19 Sebastianus A. Goorden , Jacopo Bertolotti , Allard P. Mosk

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of…

In an effort to transcend the limitations of differential imaging of exoplanets in the era of extremely large telescopes (ELTs), the first paper in this series established a rigorous, fully polarimetric framework for determining the science…

Instrumentation and Methods for Astrophysics · Physics 2016-02-18 Richard A. Frazin

Optical interferometers increasingly use single-mode fibers as spatial filters to convert varying wavefront distortion into intensity fluctuations which can be monitored for accurate calibration of fringe amplitudes. Here I propose using an…

Astrophysics · Physics 2009-11-06 J. D. Monnier

The WALOP-South instrument will be mounted on the 1 m SAAO telescope in South Africa as part of the PASIPHAE program to carry out a linear imaging polarization survey of the Galactic polar regions in the optical band. Designed to achieve…

Single-particle imaging experiments of biomolecules at x-ray free-electron lasers (XFELs) require processing of hundreds of thousands (or more) of images that contain very few x-rays. Each low-flux image of the diffraction pattern is…

Instrumentation and Detectors · Physics 2012-03-16 Hugh T. Philipp , Kartik Ayyer , Mark W. Tate , Veit Elser , Sol M. Gruner

We describe a simple but efficient method for deriving a consistent set of monopole and dipole corrections for multi-frequency sky map data sets, allowing robust parametric component separation with the same data set. The computational core…

Cosmology and Nongalactic Astrophysics · Physics 2014-12-01 I. K. Wehus , U. Fuskeland , H. K. Eriksen , A. J. Banday , C. Dickinson , T Ghosh , K. M. Gorski , C. R. Lawrence , J. P. Leahy , D. Maino , P. Reich , W. Reich

We present Magnetic Resonance Force Microscopy (MRFM) measurements of Ferromagnetic Resonance (FMR) in a 50 nm thick permalloy film, tilted with respect to the direction of the external magnetic field. At small probe-sample distances the…

Fourier Ptychographic Microscopy (FPM) is a recent technique to overcome the diffraction limit of a low numerical aperture (NA) objective lens by algorithmic post-processing of several lower resolved images. It can increase the…

We analyzed archival spectra acquired with the Hubble Space Telescope for a study of interstellar C2. Absorption from the electronic transitions, D ^1Sigma^+_u -- X ^1Sigma^+_g (0,0) as well as F ^1Pi_u -- X ^1Sigma^+_g (0,0) and (1,0), was…

Astrophysics of Galaxies · Physics 2015-06-12 Ryan C. Hupe , Y. Sheffer , S. R. Federman

Axons in white matter have been shown to have varying geometries within a bundle using ex vivo imaging techniques, but what does this mean for diffusion MRI (dMRI) based spherical deconvolution (SD)? SD attempts to estimate the fibre…

Medical Physics · Physics 2022-03-04 Ross Callaghan , Daniel C. Alexander , Marco Palombo , Hui Zhang

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of…

We investigate lensless endoscopy using coherent beam combining and aperiodic multicore fibers (MCF). We show that diffracted orders, inherent to MCF with periodically arranged cores, dramatically reduce the field of view (FoV) and that…

Single molecule localization microscopy has the potential to resolve structural details of biological samples at the nanometer length scale. However, to fully exploit the resolution it is crucial to account for the anisotropic emission…