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Spectropolarimetric observations used to infer the solar magnetic fields are obtained with a limited spatial resolution. The effects of this limited resolution on the inference of the open flux over the observed region have not been…

Solar and Stellar Astrophysics · Physics 2024-02-06 Ivan Milic , Rebecca Centeno , Xudong Sun , Matthias Rempel , Jaime de la Cruz Rodriguez

Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However,…

We derive the fundamental limit to the resolution of far-field optical imaging, and demonstrate that, while a bound to the resolution of a fundamental nature does exit, contrary to the conventional wisdom it is neither exactly equal to nor…

Optics · Physics 2019-03-14 Evgenii Narimanov

We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…

One of the major limitations of adaptive optics (AO) corrected image post-processing is the lack of knowledge on the system point spread function (PSF). The PSF is not always available as a direct imaging on isolated point like objects such…

Instrumentation and Methods for Astrophysics · Physics 2020-07-22 Romain Fétick , Laurent Mugnier , Thierry Fusco , Benoit Neichel

Diffraction-limited focusing deep into biological tissue is challenging due to spherical aberrations that lead to a broadening of the focal spot particularly in axial direction. While the diffraction-limit can be restored employing…

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Using the almost all-sky 2MASS Photometric Redshift catalogue (2MPZ) we perform for the first time a tomographic analysis of galaxy angular clustering in the local Universe ($z<0.24$). We estimate the angular auto- and cross-power spectra…

Cosmology and Nongalactic Astrophysics · Physics 2018-02-01 A. Balaguera-Antolínez , M. Bilicki , E. Branchini , A. Postiglione

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

Fibered confocal fluorescence microscopy (FCFM) is a new technique that can be used during a bronchoscopy to analyze the nature of the human bronchial and alveolar mucosa fluorescence microstructure. An endoscopic fibered confocal…

Medical Physics · Physics 2008-02-27 G. Bourg-Heckly , L. Thiberville , C. Vever-Bizet , B. Viellerobe

An undersampled point spread function may interact with the microstructure of a solid-state detector such that the total flux detected can depend sensitively on where the PSF center falls within a pixel. Such intra-pixel sensitivity…

Astrophysics · Physics 2009-10-31 Tod R. Lauer

We propose a focal-plane wavefront sensor (FPWFS) based on a short multimode fiber (MMF) capable of operating under moderately broadband illumination. By coupling the aberrated focal-plane field into an MMF of length <1 cm, we preserve…

Instrumentation and Methods for Astrophysics · Physics 2026-03-13 Auxiliadora Padrón-Brito , Natalia Arteaga-Marrero , Ian Cunnyngham , Jeff Kuhn

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…

Biological Physics · Physics 2011-12-23 Charles Edouard Leroux , Irène Wang , Jacques Derouard , Antoine Delon

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

The phasor field has been shown to be a valuable tool for non-line-of-sight imaging. We present a formal analysis of phasor-field imaging using paraxial wave optics. Then, we derive a set of propagation primitives---using the two-frequency,…

Optics · Physics 2019-07-24 Justin Dove , Jeffrey H. Shapiro

We apply state-of-the art data analysis methods to a number of fictitious CMB mapping experiments, including 1/f noise, distilling the cosmological information from time-ordered data to maps to power spectrum estimates, and find that in all…

Astrophysics · Physics 2008-11-26 Max Tegmark

Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…

Quantitative Methods · Quantitative Biology 2019-08-15 Renata Rychtarikova , Georg Steiner , Gero Kramer , Michael B. Fischer , Dalibor Stys

Point Spread Function (PSF) for imaging through inhomogeneous refractive medium, such as atmospheric turbulence is bounded by three constraints [Charnotskii, Opt. Eng., 52, 04600, (2013)]. PSF is non-negative, band-limited, and the third…

Optics · Physics 2022-07-06 Mikhail Charnotskii

For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial…

Optics · Physics 2020-01-27 Lyubov V. Amitonova , Johannes F. de Boer

I propose a spatial-mode demultiplexing (SPADE) measurement scheme for the far-field imaging of spatially incoherent optical sources. For any object too small to be resolved by direct imaging under the diffraction limit, I show that SPADE…

Optics · Physics 2017-03-01 Mankei Tsang