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Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

Focusing with a 4$\pi$ parabolic mirror allows for concentrating light from nearly the complete solid angle, whereas focusing with a single microscope objective limits the angle cone used for focusing to half solid angle at maximum.…

Quantum Physics · Physics 2017-03-01 Lucas Alber , Martin Fischer , Marianne Bader , Klaus Mantel , Markus Sondermann , Gerd Leuchs

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

Optics · Physics 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

We propose a new approach for high-contrast imaging at the diffraction limit using segmented telescopes in a modest observation bandwidth. This concept, named "spectroscopic fourth-order coronagraphy", is based on a fourth-order coronagraph…

Instrumentation and Methods for Astrophysics · Physics 2021-03-17 Taro Matsuo , Satoshi Itoh , Yuji Ikeda

Recent work has shown that label-efficient few-shot learning through self-supervision can achieve promising medical image segmentation results. However, few-shot segmentation models typically rely on prototype representations of the…

Image and Video Processing · Electrical Eng. & Systems 2022-03-07 Stine Hansen , Srishti Gautam , Robert Jenssen , Michael Kampffmeyer

Elemental mapping images can be achieved through step scanning imaging using pinhole optics or micro pore optics (MPO), or alternatively by full-field X-ray fluorescence imaging (FF-XRF). X-ray optics for FF-XRF can be manufactured with…

Image and Video Processing · Electrical Eng. & Systems 2023-01-25 Siwen An , David Krapohl , Benny Thörnberg , Romain Roudot , Emile Schyns , Börje Norlin

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Several recent designs for planet-finding telescopes use coronagraphs operating at visible wavelengths to suppress starlight along the telescope's optical axis while transmitting any off-axis light from circumstellar material. We describe a…

Astrophysics · Physics 2009-09-17 Marc J. Kuchner , Wesley A. Traub

The possibility of focusing light to an ever tighter spot has important implications for many applications and fields of optics research, such as nano-optics and plasmonics, laser-scanning microscopy, optical data storage and many more. The…

Flexible fiber-optic endoscopes provide a minimally-invasive solution for imaging at depths beyond the reach of conventional microscopes. Current endoscopes require focusing and/or scanning mechanisms at the distal end, which limit…

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

Optics · Physics 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Multimode fibers (MMFs) are attractive ultra-thin replacements for state-of-the-art endoscopes, but the phase randomization in propagation through MMFs poses a major hurdle for imaging and focusing of light. Recently, this challenge has…

Optics · Physics 2015-06-30 Shamir Rosen , Doron Gilboa , Ori Katz , Yaron Silberberg

We shape fs optical pulses and deliver them in a single spatial mode to the input of a multimode fiber. The pulse is shaped in time such that at the output of the multimode fiber an ultrashort pulse appears at a predefined focus. Our result…

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles, which plays a vital role in studying the molecular structure and dynamics of bio-complex. However, it is difficult to resolve the dipole…

We report on a novel detection system for collinear laser spectroscopy which provides an almost $4\pi$ solid angle for fluorescence photon detection by employing curved surface mirrors. Additional parabolic angular filters offer passive…

Instrumentation and Detectors · Physics 2020-07-07 Bernhard Maaß , Kristian König , Jörg Krämer , Andrew J. Miller , Kei Minamisono , Wilfried Nörtershäuser , Felix Sommer

Fluoroscopic imaging that captures X-ray images at video framerates is advantageous for guiding catheter insertions by vascular surgeons and interventional radiologists. Visualizing the dynamical movements non-invasively allows complex…

Image and Video Processing · Electrical Eng. & Systems 2020-01-06 Jacky C. K. Chow , Steven K. Boyd , Derek D. Lichti , Janet L. Ronsky

The usage of a GaAsP (gallium arsenide phosphide) photomultiplier for microscopical imaging allows the evaluation of low-light luminescent objects. We designed a setup for collecting a confocal microscopic image signal, which is divided…

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam…

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