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Fluorescent molecules emit light in a dipole radiation pattern that can be used to infer their orientation through defocused fluorescence microscopy. Proper measurement of the orientation requires mathematical modeling of the radiation…

Optics · Physics 2025-08-07 E. Dey , M. Elorza , F. W. Foss , J. J. Gomez Cadenas , B. J. P. Jones

Propagation of sound beam diffracted from a circular aperture in far-field region has been studied in this paper by the method of angular spectrum representation and stationary phase method. This nonparaxial theory is useful when beam angle…

Classical Physics · Physics 2007-05-23 Manas Kumar Roy

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Fourier ptychography (FP) imaging, drawing on the idea of synthetic aperture, has been demonstrated as a potential approach for remote sub-diffraction-limited imaging. Nevertheless, the farthest imaging distance is still limited around 10 m…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due…

Instrumentation and Detectors · Physics 2009-11-07 Masahiro Yoshita , Kazuko Koyama , Motoyoshi Baba , Hidefumi Akiyama

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang

There are several high-performance adaptive optics systems that deliver diffraction-limited imaging on ground-based telescopes, which renewed the interest of single-mode fiber (SMF) spectroscopy for exoplanet characterization. However, the…

Instrumentation and Methods for Astrophysics · Physics 2021-05-19 S. Y. Haffert

Illuminating or imaging samples from a broad angular range is essential in a wide variety of computational 3D imaging and resolution-enhancement techniques, such as optical projection tomography (OPT), optical diffraction tomography (ODT),…

We develop a new approach for studying flux anomalies in quadruply-imaged fold lens systems. We show that in the absence of substructure, microlensing, or differential absorption, the expected flux ratios of a fold pair can be tightly…

Cosmology and Nongalactic Astrophysics · Physics 2014-11-20 David M. Goldberg , Mary K. Chessey , Wendy B. Harris , Gordon T. Richards

The 21-cm global signal is obscured by very bright galactic and extra galactic foreground emissions. Typical single-spectrum fit (SSF) based methods for foreground/signal separation can result in biased estimates of the cosmological signal…

Cosmology and Nongalactic Astrophysics · Physics 2025-06-27 Yordan D. Ignatov , Jonathan R. Pritchard

The use of individual multimode optical fibers in endoscopy applications has the potential to provide highly miniaturized and noninvasive probes for microscopy and optical micromanipulation. A few different strategies have been proposed…

Future optical interferometric instrumentation mainly relies on the availability of an efficient cophasing system: once available, what has so far postponed the relevance of direct imaging with an interferometer will vanish. This paper…

Astrophysics · Physics 2008-11-26 O. Lardiere , F. Martinache , F. Patru

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

Optics · Physics 2025-06-24 Byung Gyu Chae

By switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical…

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

We present simulations of an efficient high numerical aperture solid immersion metalens concept for fluorescence microscopy. The technique exploits the preferential emission of interfacial dipoles into a high refractive index substrate…

Optics · Physics 2022-01-12 Zhiheng Li , Zequan Chen , Rupert F. Oulton , Ming Fu

High refractive index semiconductor nanowires have recently been demonstrated experimentally as an efficient platform for enhancing the signal in fluorescence-based biosensors. Here, we study through modelling how a vertical GaP nanowire…

Optics · Physics 2025-02-04 Nicklas Anttu

We report on a method to obtain confocal imaging through multimode fibers using optical correlation. First, we measure the fiber's transmission matrix in a calibration step. This allows us to create focused spots at one end of the fiber by…