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Related papers: Molecular contrast on phase-contrast microscope

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Effective and accurate in-vivo diagnosis of retinal pathologies requires high performance imaging devices, combining a large field of view and the ability to discriminate the ballistic signal from the diffuse background in order to provide…

Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images,…

Quantum simulations with ultracold atoms typically create atomic wavefunctions with structures at optical length scales, where direct imaging suffers from the diffraction limit. In analogy to advances in optical microscopy for biological…

Quantum Gases · Physics 2019-04-10 Sarthak Subhankar , Yang Wang , Tsz-Chun Tsui , Steven L. Rolston , James V. Porto

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

Quantitative Methods · Quantitative Biology 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

Quantum-optical coherence tomography (QOCT) is an optical sectioning modality based on the quantum interference of photon pairs, obtained from a spontaneous parametric downconversion (SPDC) source. The promise of QOCT derives from two…

Time resolution is one of the most severe limitations of scanning probe microscopies (SPMs), since the typical image acquisition times are in the order of several seconds or even few minutes. As a consequence, the characterization of…

Optical spectroscopy plays an essential role across scientific research and industry for non-contact materials analysis1-3, increasingly through in-situ or portable platforms4-6. However, when considering low-light-level applications,…

Optical detection of nanoscale objects without relying on fluorescence is a current challenge due to their extremely weak interaction with light. Resonator-enhanced absorption microscopy is a novel tool to heavily boost the light-matter…

The vortex coronagraph is an optical instrument that precisely removes on-axis starlight allowing for high contrast imaging at small angular separation from the star, thereby providing a crucial capability for direct detection and…

Instrumentation and Methods for Astrophysics · Physics 2015-11-04 Garreth J. Ruane , Elsa Huby , Olivier Absil , Dimitri Mawet , Christian Delacroix , Brunella Carlomagno , Grover A. Swartzlander

A device based on a three-block Fresnel zone plate interferometer is proposed for hard X-ray phase-contrast imaging. The device combines a low requirement for the coherence of the initial radiation (the interferometer operates in the…

Instrumentation and Detectors · Physics 2021-04-22 L. A. Haroutunyan

An optical microscope is probably the most intuitive, simple and commonly used instrument to observe objects and discuss behaviors through images. Although the idea of imaging electrochemical processes operando by optical microscopy was…

Optics · Physics 2022-11-16 Jean-François Lemineur , Hui Wang , Wei Wang , Frédéric Kanoufi

Low dose, high contrast x-ray imaging is of general interest in medical diagnostic applications. X-ray Mach-Zehnder interferometers using collimated synchrotron beams demonstrate the highest levels of phase contrast under a given exposure…

Modern microscopy techniques are developing towards high-resolution imaging, and tremendous progress has been made in past decades; however, the imaging of individual biological macromolecules at atomic resolution using short-wavelength…

Optics · Physics 2022-01-12 Tatiana Latychevskaia

Imaging transparent samples remains an ongoing challenge in the study of unstained biological cells and material samples. Widely used methods trade off system complexity, cost and bulk, computational efficiency and information content. Here…

Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…

Optics · Physics 2025-10-14 Guifeng Li , Chaoyang Gong

We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser-electron-interaction,…

Instrumentation and Detectors · Physics 2010-09-10 Holger Mueller , Jian Jin , Radostin Danev , John Spence , Howard Padmore , Robert M. Glaeser

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

Phase and polarisation of coherent light are highly perturbed by interaction with microstructural changes in pre-malignant tissue, holding promise for label-free early cancer detection in endoscopically accessible tissues such as the…

Metasurface lenses are miniature flat lenses that can precisely control the phase, amplitude, and polarization of incident light by modulating the parameters of each unit on the substrate. Compared with conventional optical lenses, they…

Optics · Physics 2025-07-02 Runhui Zhong , Jinzhong Ling , Yangyang Li , Xudong Yang , Xiaorui Wang

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

Optics · Physics 2013-05-30 Osip Schwartz , Dan Oron