Related papers: Molecular contrast on phase-contrast microscope
State-of-the-art microscopes use intense lasers that can severely disturb biological processes, function and viability. This introduces hard limits on performance that only quantum photon correlations can overcome. Here we demonstrate this…
We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…
Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has…
Photonic molecules, named by analogy with chemical molecules, are clusters of closely located electromagnetically interacting microcavities or "photonic atoms". As two or several microcavities are brought close together, their optical modes…
X-ray phase-contrast imaging has recently led to a revolution in resolving power and tissue contrast in biomedical imaging, microscopy and materials science. The necessary high spatial coherence is currently provided by either large-scale…
Quantum imaging is an advanced method for microscopy or investigating the optical properties of materials or bio-medical inspections with high accuracy, low noise, and extremely low photo-damage. In previous work, we proposed a quantum…
Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…
Single atoms can be considered as basic objects for electron microscopy to test the microscope performance and basic concepts for modeling of image contrast. In this work high-resolution transmission electron microscopy was applied to image…
We propose and experimentally demonstrate a differential microscopy method to obtain simultaneous amplitude, phase, and quantitative polarization gradient imaging in a single experimental embodiment. A full-field optical spatial…
Interferometric scattering microscopy was widely applied in nanoscopic detection and tracking due to its high sensitivity and label-free manner. The sensitivity is limited by contrast. Oblique illumination provided high contrast with lower…
Optical imaging of metabolism can provide key information about health and disease progression in cells and tissues, however, current methods have lacked gold-standard information about histological structure. Conversely, histology and…
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and…
As a revolutionary observation tool in life science, biomedical, and material science, optical microscopy allows imaging of samples with high spatial resolution and a wide field of view. However, conventional microscopy methods are limited…
A phase-sensitive microscopy technique is proposed and demonstrated that employs the momentum correlations inherent in spontaneous parametric down-conversion. One photon from a correlated pair is focused onto a microscopic target while the…
Phase-contrast imaging is an emerging technology that may increase the signal-difference-to-noise ratio in medical imaging. One of the most promising phase-contrast techniques is Talbot interferometry, which, combined with energy-sensitive…
Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution…
An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…
The technological progress in spatial-light modulators (SLM) technology has made it possible to use those devices as programmable active focal-plane phase coronagraphic masks, opening the door to novel versatile and adaptive high-contrast…
High-contrast observations in optical and infrared astronomy are defined as any observation requiring a technique to reveal a celestial object of interest that is in such close angular proximity to another source brighter by a factor of at…
Polarizing microscopy brought about many advancements in the science of liquid crystals and other soft materials, including those of biological origin. Recent developments in optics and computer-based analysis enabled a new generation of…