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Related papers: Virtualization of tissue staining in digital patho…

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In digital pathology, many image analysis tasks are challenged by the need for large and time-consuming manual data annotations to cope with various sources of variability in the image domain. Unsupervised domain adaptation based on…

Image and Video Processing · Electrical Eng. & Systems 2022-05-18 Nassim Bouteldja , Barbara Mara Klinkhammer , Tarek Schlaich , Peter Boor , Dorit Merhof

Histopathological analysis is crucial in artery characterization for coronary artery disease (CAD). However, histology requires an invasive and time-consuming process. In this paper, we propose to generate virtual histology staining using…

Image and Video Processing · Electrical Eng. & Systems 2022-11-15 Xueshen Li , Hongshan Liu , Xiaoyu Song , Brigitta C. Brott , Silvio H. Litovsky , Yu Gan

Double staining in histopathology, particularly for metaplastic breast cancer, typically employs H&E and P63 dyes. However, P63's tissue damage and high cost necessitate alternative methods. This study introduces xAI-CycleGAN, an advanced…

Image and Video Processing · Electrical Eng. & Systems 2023-12-07 Tibor Sloboda , Lukáš Hudec , Wanda Benešová

Immunohistochemical (IHC) stains play a vital role in a pathologist's analysis of medical images, providing crucial diagnostic information for various diseases. Virtual staining from hematoxylin and eosin (H&E)-stained whole slide images…

Image and Video Processing · Electrical Eng. & Systems 2024-10-03 Sitong Liu , Kechun Liu , Samuel Margolis , Wenjun Wu , Stevan R. Knezevich , David E Elder , Megan M. Eguchi , Joann G Elmore , Linda Shapiro

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Histopathological staining of human tissue is essential for disease diagnosis. Recent advances in virtual tissue staining technologies using artificial intelligence (AI) alleviate some of the costly and tedious steps involved in traditional…

Image and Video Processing · Electrical Eng. & Systems 2025-06-17 Luzhe Huang , Yuzhu Li , Nir Pillar , Tal Keidar Haran , William Dean Wallace , Aydogan Ozcan

Virtual staining has emerged as a powerful alternative to traditional histopathological staining techniques, enabling rapid, reagent-free image transformations. However, existing evaluation methods predominantly rely on full-reference image…

Image and Video Processing · Electrical Eng. & Systems 2025-07-18 Qiankai Wang , James E. D. Tweel , Parsin Haji Reza , Anita Layton

Conventional hematoxylin-eosin (H&E) staining is limited to revealing cell morphology and distribution, whereas immunohistochemical (IHC) staining provides precise and specific visualization of protein activation at the molecular level.…

Image and Video Processing · Electrical Eng. & Systems 2024-07-30 Fuqiang Chen , Ranran Zhang , Boyun Zheng , Yiwen Sun , Jiahui He , Wenjian Qin

Virtual staining of tissue offers a powerful tool for transforming label-free microscopy images of unstained tissue into equivalents of histochemically stained samples. This study presents a diffusion model-based super-resolution virtual…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Hanlong Chen , Aydogan Ozcan

The process of digitising histology slides involves multiple factors that can affect a whole slide image's (WSI) final appearance, including the staining protocol, scanner, and tissue type. This variability constitutes a domain shift and…

Computer Vision and Pattern Recognition · Computer Science 2024-11-26 Manahil Raza , Saad Bashir , Talha Qaiser , Nasir Rajpoot

With the rapid development of digital pathology, virtual staining has become a key technology in multimedia medical information systems, offering new possibilities for the analysis and diagnosis of pathological images. However, existing…

Computer Vision and Pattern Recognition · Computer Science 2025-06-10 Hao Yang , JianYu Wu , Run Fang , Xuelian Zhao , Yuan Ji , Zhiyu Chen , Guibin He , Junceng Guo , Yang Liu , Xinhua Zeng

In the clinic, resected tissue samples are stained with Hematoxylin-and-Eosin (H&E) and/or Immunhistochemistry (IHC) stains and presented to the pathologists on glass slides or as digital scans for diagnosis and assessment of disease…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Parmida Ghahremani , Joseph Marino , Ricardo Dodds , Saad Nadeem

Chemical staining methods are dependable but require extensive time, expensive chemicals, and raise environmental concerns. These challenges highlight the need for alternative solutions like virtual staining, which accelerates the…

Image and Video Processing · Electrical Eng. & Systems 2024-07-02 Mehdi Ounissi , Ilias Sarbout , Jean-Pierre Hugot , Christine Martinez-Vinson , Dominique Berrebi , Daniel Racoceanu

Organ transplantation serves as the primary therapeutic strategy for end-stage organ failures. However, allograft rejection is a common complication of organ transplantation. Histological assessment is essential for the timely detection and…

Histopathology images; microscopy images of stained tissue biopsies contain fundamental prognostic information that forms the foundation of pathological analysis and diagnostic medicine. However, diagnostics from histopathology images…

Computer Vision and Pattern Recognition · Computer Science 2019-10-30 Aïcha BenTaieb , Ghassan Hamarneh

An optical microscopic examination of thinly cut stained tissue on glass slides prepared from a FFPE tissue blocks is the gold standard for tissue diagnostics. In addition, the diagnostic abilities and expertise of any pathologist is…

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Medical practitioners use a number of diagnostic tests to make a reliable diagnosis. Traditionally, Haematoxylin and Eosin (H&E) stained glass slides have been used for cancer diagnosis and tumor detection. However, recently a variety of…

Image and Video Processing · Electrical Eng. & Systems 2023-12-27 Abubakr Shafique , Morteza Babaie , Ricardo Gonzalez , H. R. Tizhoosh