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Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…

Random scattering is usually viewed as a serious nuisance in optical imaging, and needs to be prevented in the conventional imaging scheme based on single-photon interference. Here we proposed a two-photon imaging scheme with the widely…

Optics · Physics 2018-09-25 Peilong Hong

We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During…

Optical imaging deep inside scattering media remains a fundamental problem in bio-imaging. While wavefront shaping has been shown to allow focusing of coherent light at depth, achieving it non-invasively remains a challenge. Various…

Optics · Physics 2019-12-24 Antoine Boniface , Baptiste Blochet , Jonathan Dong , Sylvain Gigan

We present a widefield two-dimensional electronic spectroscopy microscope (2DESM) that integrates multidimensional coherent spectroscopy with optical imaging, enabling femtosecond temporal and micrometer spatial resolution. The broadband…

Optical detection of nanoscale objects without relying on fluorescence is a current challenge due to their extremely weak interaction with light. Resonator-enhanced absorption microscopy is a novel tool to heavily boost the light-matter…

Optical micro-spectroscopy is an invaluable tool for studying and characterizing samples ranging from classical semiconductors to low-dimensional materials and heterostructures. To date, most implementations are based on point-scanning…

Current optoacoustic microscopy configurations often have narrow focal ranges that limit their use for fast volumetric imaging applications. Here, the focal range of optoacoustic microscopes is extended by matching the elongated optical…

Optics · Physics 2021-04-15 Zakiullah Ali , Christian Zakian , Vasilis Ntziachristos

We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

By exploiting the quantised nature of light, we demonstrate a sub-shot-noise scanning optical transmittance microscope. Our microscope demonstrates, with micron scale resolution, a factor of improvement in precision of 1.76(9) in…

Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded either with spectral or temporal resolution. Thanks to…

Optics · Physics 2018-06-08 Mickael Mounaix , Duc Minh Ta , Sylvain Gigan

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

We introduce a novel method for creating a high-quality, sharply defined, non-diffracting optical bottle beam by focusing a Bessel beam propagating through a flat multi-level diffractive lens (MDL). This study highlights the impact of the…

Intrabeam scattering (IBS) is a fundamental effect that can limit the performance of high-brightness electron machines, yet it has so far been neglected in standard modelling of RF photoinjectors. Recent measurements at SwissFEL reveal that…

Accelerator Physics · Physics 2025-10-03 Thomas Geoffrey Lucas , Paolo Craievich , Eduard Prat , Sven Reiche , Erion Gjonaj

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications…