English
Related papers

Related papers: Subnanometer localization accuracy in widefield op…

200 papers

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

Proper confidence calibration of deep neural networks is essential for reliable predictions in safety-critical tasks. Miscalibration can lead to model over-confidence and/or under-confidence; i.e., the model's confidence in its prediction…

Machine Learning · Computer Science 2023-08-08 Shuang Ao , Stefan Rueger , Advaith Siddharthan

The availability of positional information is of great importance in many commercial, public safety, and military applications. The coming years will see the emergence of location-aware networks with sub-meter accuracy, relying on accurate…

Information Theory · Computer Science 2010-06-07 Yuan Shen , Moe Z. Win

With model trustworthiness being crucial for sensitive real-world applications, practitioners are putting more and more focus on improving the uncertainty calibration of deep neural networks. Calibration errors are designed to quantify the…

Machine Learning · Computer Science 2024-03-14 Sebastian G. Gruber , Florian Buettner

The precise determination of the position of point-like emitters and scatterers using far-field optical imaging techniques is of utmost importance for a wide range of applications in medicine, biology, astronomy, and physics. Although the…

In recent years several methods to overcome diffraction limit in the far field microscopy have been demonstrated. Still the problem of superresolution is reliably solved only for fluorescent microscopy, giving a resolution of up to 20-30nm.…

Optics · Physics 2013-09-03 Yu. V. Miklyaev , S. A. Asselborn , K. A. Zaytsev , M. Ya. Darscht

Precision is a virtue throughout science in general and in optics in particular where carefully fabricated nanometer-scale devices hold great promise for both classical and quantum photonics [1-6]. In such nanostructures, unavoidable…

We show why and when optics needs thickness as well as width or area. Wave diffraction explains the fundamental need for area or diameter of a lens or aperture to achieve some resolution or number of pixels in microscopes and cameras. Now…

Optics · Physics 2023-01-09 David. A. B. Miller

Robustly and accurately localizing objects in real-world environments can be challenging due to noisy data, hardware limitations, and the inherent randomness of physical systems. To account for these factors, existing works estimate the…

Computer Vision and Pattern Recognition · Computer Science 2023-09-19 Moussa Kassem Sbeyti , Michelle Karg , Christian Wirth , Azarm Nowzad , Sahin Albayrak

One of the main limitations for the resolution of optical instruments is the size of the sensor's pixels. In this paper we introduce a new sub pixel resolution algorithm to enhance the resolution of images. This method is based on the…

Instrumentation and Detectors · Physics 2012-11-12 Siamak Khademi , Ahmad Darudi , Zahra Abbasi

Optical interferometers provide multiple wavelength measurements. In order to fully exploit the spectral and spatial resolution of these instruments, new algorithms for image reconstruction have to be developed. Early attempts to deal with…

Instrumentation and Methods for Astrophysics · Physics 2015-06-11 Éric Thiébaut , Ferréol Soulez , Loïc Denis

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

Optics · Physics 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky

Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

The ability to spatially and temporally map nanoscale environments in situ over extended timescales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins,…

Optics · Physics 2023-10-04 Guangjie Cui , Do Young Kim , Di Zu , Guanbo Chai , Somin Eunice Lee

We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solution gives the maximum likelihood estimator of multi-emitter…

Optics · Physics 2015-12-21 Yuto Ashida , Masahito Ueda

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

In this chapter we present a brief summary of methods, instruments and calibration techniques used in modern astronomical polarimetry in the optical wavelengths. We describe the properties of various polarization devices and detectors used…

Instrumentation and Methods for Astrophysics · Physics 2019-08-29 Andrei Berdyugin , Vilppu Piirola , Juri Poutanen

A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…

Optics · Physics 2010-08-27 Alexander R. Small

Depth estimation is a fundamental problem for light field photography applications. Numerous methods have been proposed in recent years, which either focus on crafting cost terms for more robust matching, or on analyzing the geometry of…

Computer Vision and Pattern Recognition · Computer Science 2018-07-27 Jie Chen , Junhui Hou , Yun Ni , Lap-Pui Chau

Accurately measuring the size, morphology, and structure of nanoparticles is very important, because they are strongly dependent on their properties for many applications. In this paper, we present a deep-learning based method for…

Materials Science · Physics 2022-07-29 Claudius Zelenka , Marius Kamp , Kolja Strohm , Akram Kadoura , Jacob Johny , Reinhard Koch , Lorenz Kienle