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Super-resolution microscopy is providing unprecedented insights into biology by resolving details much below the diffraction limit. State-of-the-art Single Molecule Localization Microscopy (SMLM) techniques for super-resolution are…

Quantitative Methods · Quantitative Biology 2016-12-13 Krishna Agarwal , Radek Macháň

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

We introduce a wide field hyperspectral microscope using the Fourier-transform approach. The interferometer is based on the Translating-Wedge-Based Identical Pulses eNcoding System (TWINS) [Opt. Lett. 37, 3027 (2012)], a common-path…

Instrumentation and Detectors · Physics 2019-09-26 A. Candeo , B. E. Nogueira de Faria , M. Erreni , G. Valentini , A. Bassi , A. M. de Paula , G. Cerullo , C. Manzoni

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang

Light detection and ranging (LiDAR) has been widely used in autonomous driving and large-scale manufacturing. Although state-of-the-art scanning LiDAR can perform long-range three-dimensional imaging, the frame rate is limited by both…

Optics · Physics 2021-03-10 Zihan Zang , Zhi Li , Yi Luo , Yanjun Han , Xuanyi Liu , H. Y. Fu

Rapid and programmable shaping of light fields is central to modern microscopy, display technologies, optical communications and sensing, quantum engineering, and quantum information processing. Current wavefront shaping technologies face a…

Quantum Physics · Physics 2026-01-15 Xin Wei , Zeyang Li , Abhishek V. Karve , Adam L. Shaw , David I. Schuster , Jonathan Simon

Ultrafast imaging is a powerful tool for studying space-time dynamics in photonic material, plasma physics, living cells, and neural activity. Pushing the imaging speed to the quantum limit could reveal extraordinary scenes about the…

Instrumentation and Detectors · Physics 2018-07-03 Xuanke Zeng , Yi Cai , Shuiqin Zheng , Shixiang Xu , Hu Long , Xiaowei Lu , Xiuwen Zhang , Weixin Xie , Jingzhen Li

Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or…

Raman sensing and Raman microscopy are amongst the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging with molecular contrast. However, the high cost and…

High-speed imaging of cells in flow is essential for probing cellular heterogeneity in large populations. Existing imaging approaches based on single-pixel detection and spatio-temporal encoding provide exceptional speed, but typically rely…

Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…

Fast-responding detector arrays are commonly used for imaging rapidly-changing scenes. Besides array detectors, a single-pixel detector combined with a broadband optical spectrum can also be used for rapid imaging by mapping the spectrum…

Optics · Physics 2019-09-04 Chengying Bao , Myoung-Gyun Suh , Kerry Vahala

Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly due to the…

Endoscopic imaging is essential for real-time visualization of internal organs, yet conventional systems remain bulky, complex, and expensive due to their reliance on large, multi-element optical components. This limits their accessibility…

A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well-defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and…

Biological Physics · Physics 2017-11-10 Shay Ohayon , Antonio Miguel Caravaca-Aguirre , Rafael Piestun , James J. DiCarlo

Biological and biomedical samples are routinely examined using focused two-photon (2P) fluorescence microscopy due to its intrinsic axial sectioning and reduced out-of-focus bleaching. However, 2P imaging often requires excitation…

Infrared (IR) imaging has become a viable tool for visualizing various chemical bonds in a specimen. The performance, however, is limited in terms of spatial resolution and imaging speed. Here, instead of measuring the loss of the IR beam,…

Optics · Physics 2018-11-20 Yeran Bai , Delong Zhang , Lu Lan , Yimin Huang , Kerry Maize , Ali Shakouri , Ji-Xin Cheng
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