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Related papers: Spectro-temporal encoded Multiphoton Microscopy

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Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Imaging through a strongly diffusive medium remains an outstanding challenge in particular in association with applications in biological and medical imaging. Here we propose a method based on a single-photon time-of-flight camera that…

We introduce a new parallelized approach to photothermal microscopy that utilizes mid-infrared dual-comb lasers, enabling simultaneous measurements at hundreds of wavelengths. This technology allows for high-speed, label-free chemical…

High-speed mid-infrared (MIR) videography constitutes an enabling tool to monitor and analyze various dynamics in scientific research and industrial applications, such as combustion diagnostics, explosion reactions, photosynthetic tracking,…

Optics · Physics 2026-05-28 Ruiyang Qin , Kun Huang , Min Peng , Jianan Fang , Ben Sun , Zhengru Guo , Heping Zeng

Rigid endoscopes like graded-index (GRIN) lenses are known tools in biological imaging, but it is conceptually difficult to miniaturize them. In this letter, we demonstrate an ultra-thin rigid endoscope with a diameter of only 125 microns.…

RAndom Temporal Signals (RATS) method has proven to be a useful and versatile method for measuring photoluminescence (PL) dynamics and fluorescence lifetime imaging (FLIM). Here, we present two fundamental development steps in the method.…

Optics · Physics 2023-02-15 Jiri Junek , Karel Zidek

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

Optics · Physics 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Accurate and fast characterization of spatiotemporal information of high-intensity, ultrashort pulses is crucial in the field of strong-field laser science and technology. While conventional self-referenced interferometers were widely used…

Optics · Physics 2023-07-19 Yifei Chen , Zhiyuan Huang , Ding Wang , Yu Zhao , Jianhua Fu , Meng Pang , Yuxin Leng , Zhizhan Xu

A femtosecond frequency-doubled Erbium-doped fiber laser with adjustable pulse repetition rate is developed and applied in two-photon excited fluorescence microscopy. The all-fiber laser system provides the fundamental pulse at 1560 nm…

Tissue oxygenation and perfusion can be an indicator for organ viability during minimally invasive surgery, for example allowing real-time assessment of tissue perfusion and oxygen saturation. Multispectral imaging is an optical modality…

Computer Vision and Pattern Recognition · Computer Science 2017-06-23 Geoffrey Jones , Neil T Clancy , Xiaofei Du , Maria Robu , Simon Arridge , Daniel S Elson , Danail Stoyanov

Background-free fluorescence detection in multidimensional electronic spectroscopy promises high sensitivity compared to conventional approaches. Here we explore the sensitivity limits of multidimensional electronic spectroscopy. We present…

Chemical Physics · Physics 2022-10-18 Amitav Sahu , Vivek N. Bhat , Sanjoy Patra , Vivek Tiwari

Atomic Force Microscopy - Infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared spectral region with a spatial resolution better than 50 nm. We show that the high spatial resolution can be used to perform…

Quantitative Methods · Quantitative Biology 2017-04-06 Luca Quaroni , Katarzyna Pogoda , Joanna-Wiltowska Zuber , Wojciech Kwiatek

Using an Electrically Focus Tunable Lens (EFTL), an integrating sphere and a tunable femtosecond-pulse laser (Mai Tai HP), we were able to measure the degenerate two-photon absorption coefficient (in transmission) of CdS and ZnSe in a long…

Instrumentation and Detectors · Physics 2019-09-27 Hernando Garcia , Juan Serna , Edgar Rueda

Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications…

We have developed a hyperspectral imaging scheme that involves a combination of dual-comb spectroscopy and Hadamard-transform-based single-pixel imaging. The scheme enables us to obtain 12,000 hyperspectral images of amplitude and phase at…

We demonstrate rapid imaging based on four-wave mixing (FWM) by assessing the quality of advanced materials through measurement of their nonlinear response, exciton dephasing, and exciton lifetimes. We use a WSe$_2$ monolayer grown by…

Extracting reliable and quantitative microstructure information of living tissue by non-invasive imaging is an outstanding challenge for understanding disease mechanisms and allowing early stage diagnosis of pathologies. Magnetic Resonance…

Medical Physics · Physics 2021-07-12 Milena Capiglioni , Analia Zwick , Pablo Jimenez , Gonzalo A. Alvarez

We demonstrate an approach that allows taking videos at very high-speeds of over 100,000 frames per second (fps) by exploiting the fast sampling rate of the standard rolling-shutter readout mechanism, common to most conventional sensors,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Gil Weinberg , Ori Katz

The HIgh-Time resolution (HIT) mode of FORS2 has 3 sub-modes that allow for imaging and spectroscopy over a range of timescales from milliseconds up to seconds. It is the only high time resolution spectroscopy mode available on an 8m class…

Astrophysics · Physics 2015-05-13 Kieran O'Brien

Compressive Raman is a recent framework that allows for large data compression of microspectroscopy during its measurement. Because of its inherent multiplexing architecture, it has shown imaging speeds considerably higher than conventional…