The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or amplitude-modulated light. In this paper, we examine the limitations of an innovative method of fluorescence lifetime using the strong time-frequency correlation of entangled photons generated by a continuous-wave source. For this purpose, we investigate the lifetime of IR-140 to demonstrate the functional principle and its dependencies on different experimental parameters. We also compare this technique with state-of-the-art FLIM and observed an improved figure-of-merit. Finally, we discuss the potential of a quantum advantage.
@article{arxiv.2501.08870,
title = {Benchmarking of Fluorescence Lifetime Measurements using Time-Frequency Correlated Photons},
author = {Tobias Bernd Gäbler and Nitish Jain and Patrick Then and Christian Eggeling and Markus Gräfe and Valerio Flavio Gili},
journal= {arXiv preprint arXiv:2501.08870},
year = {2025}
}