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Fluorescence lifetime experiments are a standard approach for measuring excited state dynamics and local environment effects. Here, we show that entangled photon pairs produced from a continuous-wave (CW) laser diode can replicate pulsed…

Quantum Physics · Physics 2023-05-15 Nathan Harper , Bryce P. Hickam , Manni He , Scott K. Cushing

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…

Biological Physics · Physics 2023-10-27 Pavel Malý , Dita Strachotová , Aleš Holoubek , Petr Heřman

Recent theoretical studies in quantum spectroscopy have emphasized the potential of non-classical correlations in entangled photon pairs for selectively targeting specific nonlinear optical processes in nonlinear optical responses. However,…

Chemical Physics · Physics 2026-04-21 Yuta Fujihashi , Ozora Iso , Ryosuke Shimizu , Akihito Ishizaki

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up. With modern instrumentation this limitation can be lifted significantly but some…

Data Analysis, Statistics and Probability · Physics 2018-05-23 Matthias Patting , Paja Reisch , Marcus Sackrow , Rhys Dowler , Marcelle Koenig , Michael Wahl

Measuring the lifetime of fluorescent emitters by time-correlated single photon counting (TCSPC) is a routine procedure in many research areas spanning from nanophotonics to biology. The precision of such measurement depends on the number…

Instrumentation and Detectors · Physics 2019-07-18 Dorian Bouchet , Valentina Krachmalnicoff , Ignacio Izeddin

We study a device composed of an optical interferometer integrated with a ferri-magnetic sphere resonator (FSR). Magneto-optic coupling can be employed in such a device to manipulate entanglement between optical pulses that are injected…

Quantum Physics · Physics 2022-02-02 Eyal Buks , Banoj Kumar Nayak

Frequency-filtered photon correlations have been proven to be extremely useful in grasping how the detection process alters photon statistics. Harnessing the spectral correlations also permits refinement of the emission and unraveling of…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

We present a technique to completely characterise the spectral-temporal wave function of a broadband photon pair using spectral-shearing interferometry. Our method is fully self-referencing and is generalisable across a wide range of…

Quantum Physics · Physics 2018-09-12 Alex O. C. Davis , Valerian Thiel , Brian J. Smith

A common challenge in quantum information processing with photons is the limited ability to manipulate and measure correlated states. An example is the inability to measure picosecond scale temporal correlations of a multi-photon state,…

We describe a method to probe the spectral fluctuations of a transition over broad ranges of frequencies and timescales with the high spectral resolution of Fourier spectroscopy, and a temporal resolution as high as the excited state…

We propose, analyze, and evaluate a technique for the joint measurement of time-frequency entanglement between two photons. In particular, we show that the frequency sum and time difference of two photons could be simultaneously measured…

Quantum Physics · Physics 2020-06-15 Han Liu , Amr S. Helmy

Classical time-resolved optical spectroscopy experiments are performed using sequences of ultrashort light pulses, with photon fluxes incident on the sample which are many orders of magnitude higher than real-world conditions corresponding…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

Frequency-bin entangled photons can be efficiently produced on-chip which offers a scalable, robust and low-footprint platform for quantum communication, particularly well-suited for resource-constrained settings such as mobile or…

We propose an alternative formulation of the sensor method presented in [Phys. Rev. Lett 109, 183601 (2012)] for the calculation of frequency-filtered and time-resolved photon correlations. Our approach is based on an algebraic expansion of…

Quantum Physics · Physics 2018-12-26 David I. H. Holdaway , Valentina Notararigo , Alexandra Olaya-Castro
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