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The spatial resolution of images of living samples obtained by fluorescence microscopes is physically limited due to the diffraction of visible light, which makes the study of entities of size less than the diffraction barrier (around 200…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Vasiliki Stergiopoulou , Subhadip Mukherjee , Luca Calatroni , Laure Blanc-Féraud

White light scanning interference (WLSI) microscopes provide an accurate surface topography of engineered surfaces. However, the measurement accuracy is substantially reduced in surfaces with low-reflectivity regions or high roughness, like…

Active range sensing using structured-light is the most accurate and reliable method for obtaining 3D information. However, most of the work has been limited to range sensing of static objects, and range sensing of dynamic (moving or…

Computer Vision and Pattern Recognition · Computer Science 2015-09-15 Changsoo Je , Sang Wook Lee , Rae-Hong Park

Super-resolution microscopy in the visible is an established powerful tool in several disciplines. In the infrared (IR) spectral range, however, no comparable schemes have been demonstrated to date. In this work, we experimentally…

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

We study the inverse problem of Coded Aperture Snapshot Spectral Imaging (CASSI), which captures a spatial-spectral data cube using snapshot 2D measurements and uses algorithms to reconstruct 3D hyperspectral images (HSI). However, current…

Image and Video Processing · Electrical Eng. & Systems 2024-06-19 Jincheng Yang , Lishun Wang , Miao Cao , Huan Wang , Yinping Zhao , Xin Yuan

Functional magnetic resonance imaging (fMRI) based image reconstruction plays a pivotal role in decoding human perception, with applications in neuroscience and brain-computer interfaces. While recent advancements in deep learning and…

Computer Vision and Pattern Recognition · Computer Science 2025-02-25 Weiyu Guo , Guoying Sun , JianXiang He , Tong Shao , Shaoguang Wang , Ziyang Chen , Meisheng Hong , Ying Sun , Hui Xiong

Decoding visual stimuli from neural responses recorded by functional Magnetic Resonance Imaging (fMRI) presents an intriguing intersection between cognitive neuroscience and machine learning, promising advancements in understanding human…

Computer Vision and Pattern Recognition · Computer Science 2023-12-29 Jingyuan Sun , Mingxiao Li , Zijiao Chen , Yunhao Zhang , Shaonan Wang , Marie-Francine Moens

In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly…

Image and Video Processing · Electrical Eng. & Systems 2021-10-22 Daniele Ancora , Gianluca Valentini , Antonio Pifferi , Andrea Bassi

4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…

Image and Video Processing · Electrical Eng. & Systems 2026-02-17 Cunmin Zhao , Ziyuan Luo , Guoye Guan , Zelin Li , Yiming Ma , Zhongying Zhao , Renjie Wan

An ideal imaging system provides a spatial resolution that is ultimately dictated by the numerical aperture (NA) of the illumination and collection optics. In biological tissue, resolution is further affected by scattering limiting the…

Magnetic resonance spectroscopic imaging (SI) is a unique imaging technique that provides biochemical information from in vivo tissues. The 1H spectra acquired from several spatial regions are quantified to yield metabolite concentrations…

Medical Physics · Physics 2019-10-23 Zohaib Iqbal , Dan Nguyen , Gilbert Hangel , Stanislav Motyka , Wolfgang Bogner , Steve Jiang

Three-dimensional fluorescence microscopy often suffers from anisotropy, where the resolution along the axial direction is lower than that within the lateral imaging plane. We address this issue by presenting Dual-Cycle, a new framework for…

Image and Video Processing · Electrical Eng. & Systems 2022-09-26 Tomas Kerepecky , Jiaming Liu , Xue Wen Ng , David W. Piston , Ulugbek S. Kamilov

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

Optics · Physics 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

Structured illumination (SI) enhances image resolution and contrast by projecting patterned light onto a sample. In two-phase optical-sectioning SI (OS-SI), reduced acquisition time introduces residual artifacts that conventional denoising…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Allison Davis , Yezhi Shen , Xiaoyu Ji , Fengqing Zhu

Structured illumination has been widely used for optical sectioning and 3D surface recovery. In a typical implementation, multiple images under non-uniform pattern illumination are used to recover a single object section. Axial scanning of…

Computer Vision and Pattern Recognition · Computer Science 2019-04-26 Shaowei Jiang , Jun Liao , Zichao Bian , Pengming Song , Garrett Soler , Kazunori Hoshino , Guoan Zheng

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

We introduce a single-frame diffractive imaging method called randomized probe imaging (RPI). In RPI, a sample is illuminated by a structured probe field containing speckles smaller than the sample's typical feature size. Quantitative…