Beyond multi-view deconvolution for inherently-aligned fluorescence tomography
Image and Video Processing
2021-10-22 v1 Computational Physics
Medical Physics
Optics
Abstract
In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly to the formation of inherently aligned tomographies. Our method generates sharp reconstructions, with the same accuracy reachable after sub-pixel alignment but with improved point-spread-function. The procedure can be performed simultaneously with deconvolution further increasing the reconstruction resolution.
Cite
@article{arxiv.2102.08177,
title = {Beyond multi-view deconvolution for inherently-aligned fluorescence tomography},
author = {Daniele Ancora and Gianluca Valentini and Antonio Pifferi and Andrea Bassi},
journal= {arXiv preprint arXiv:2102.08177},
year = {2021}
}