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Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

We present an advanced quantum super-resolution imaging technique based on photon statistics measurement and its accurate modeling. Our reconstruction algorithm adapts to any kind of non-Poissonian emitters, outperforming the corresponding…

Addressing the issues of severe noise and high frequency structural degradation in visible images under low-light conditions, this paper proposes a Near Infrared (NIR) aided low light image restoration method based on Frequency Decoupled…

Computer Vision and Pattern Recognition · Computer Science 2026-04-21 Ligen Shi , Zengyu Pang , Chang Liu , Shuchen Sun , Jun Qiu

Hyperspectral Imaging (HSI) for fluorescence-guided brain tumor resection enables visualization of differences between tissues that are not distinguishable to humans. This augmentation can maximize brain tumor resection, improving patient…

Image and Video Processing · Electrical Eng. & Systems 2024-02-07 David Black , Jaidev Gill , Andrew Xie , Benoit Liquet , Antonio Di leva , Walter Stummer , Eric Suero Molina

Super-resolution fluorescence microscopy, with a resolution beyond the diffraction limit of light, has become an indispensable tool to directly visualize biological structures in living cells at a nanometer-scale resolution. Despite…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Yu Li , Fan Xu , Fa Zhang , Pingyong Xu , Mingshu Zhang , Ming Fan , Lihua Li , Xin Gao , Renmin Han

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Confocal microscopy, a critical advancement in optical imaging, is widely applied because of its excellent anti-noise ability. However, it has low imaging efficiency and can cause phototoxicity. Optical-sectioning structured illumination…

Optics · Physics 2024-05-27 Weishuai Zhou , Manhong Yao , Xi Lin , Quan Yu , Junzheng Peng , Jingang Zhong

A resolution-enhanced dark-field microscope by structured light illumination is proposed to improve resolution and contrast. A set of phase-shifted fringes are projected to the sample plane at large angle to capture modulated dark-field…

Image and Video Processing · Electrical Eng. & Systems 2022-02-14 Shaobai Li , Bofan Song , Rongguang Liang

We introduce a compact, two-camera laparoscope that combines active stereo depth estimation and speckle-illumination spatial frequency domain imaging (si-SFDI) to map profile-corrected, pixel-level absorption and reduced scattering optical…

Optics · Physics 2025-01-27 Anthony A. Song , Mason T. Chen , Taylor L. Bobrow , Nicholas J. Durr

This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…

Optics · Physics 2023-12-25 Tamme Wollweber , Kartik Ayyer

Fourier single-pixel imaging (FSI) has proven capable of reconstructing high-quality two-dimensional and three-dimensional images. The utilization of the sparsity of natural images in Fourier domain allows high-resolution images to be…

Computer Vision and Pattern Recognition · Computer Science 2016-12-12 Zibang Zhang , Xueying Wang , Jingang Zhong

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…

Optics · Physics 2021-07-28 Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Ptychography is an attractive advance of coherent diffraction imaging (CDI), which can provide high lateral resolution and wide field of view. The theoretical resolution of ptychography is dose-limited, therefore making ptychography…

Biological Physics · Physics 2025-01-09 Huixiang Lin , Fucai Zhang

Magnetic Resonance Imaging (MRI) produces excellent soft tissue contrast, albeit it is an inherently slow imaging modality. Promising deep learning methods have recently been proposed to reconstruct accelerated MRI scans. However, existing…

Image and Video Processing · Electrical Eng. & Systems 2024-04-17 Yilmaz Korkmaz , Tolga Cukur , Vishal M. Patel

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…