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Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

Optics · Physics 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

Functional Spectral Imaging (FSI) models image formation as the recovery of tissue surrogates such as density and stiffness from spectral perturbations of a self-adjoint elliptic operator. Rather than relying on reflectivity or relaxation…

Medical Physics · Physics 2025-10-21 Cesar Mello Fernando Medina da Cunha

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

Limited by imaging systems, the reconstruction of Magnetic Resonance Imaging (MRI) images from partial measurement is essential to medical imaging research. Benefiting from the diverse and complementary information of multi-contrast MR…

Image and Video Processing · Electrical Eng. & Systems 2023-07-11 Jiamiao Zhang , Yichen Chi , Jun Lyu , Wenming Yang , Yapeng Tian

We present a novel illumination control technique for optical diffraction tomography (ODT). Various spatial frequencies of beam illumination were controlled by displaying time-averaged sinusoidal patterns using a digital micromirror device…

Biological Physics · Physics 2017-04-05 Kyeoreh Lee , Kyoohyun Kim , Geon Kim , Seungwoo Shin , YongKeun Park

White Light Interferometry (WLI) is a precise optical tool for measuring the 3D topography of microstructures. However, conventional WLI cannot capture the natural color of a sample's surface, which is essential for many microscale research…

Computer Vision and Pattern Recognition · Computer Science 2025-01-17 Shuo Chen , Yijin Li , Guofeng Zhang

Recently it was reported that deeply subwavelength features of free space superoscillatory electromagnetic fields can be observed experimentally and used in optical metrology with nanoscale resolution [Science 364, 771 (2019)]. Here we…

Optics · Physics 2019-10-08 T. Pu , V. Savinov , G. Yuan , N. Papasimakis , N. I. Zheludev

Magnetic Resonance Imaging (MRI) is a powerful imaging technique widely used for visualizing structures within the human body and in other fields such as plant sciences. However, there is a demand to develop fast 3D-MRI reconstruction…

Image and Video Processing · Electrical Eng. & Systems 2025-12-09 Arya Bangun , Zhuo Cao , Alessio Quercia , Hanno Scharr , Elisabeth Pfaehler

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Coherent Diffraction Imaging (CDI), a technique where an object is reconstructed from a single (2D or 3D) diffraction pattern, recovers the lost diffraction phases without a priori knowledge of the extent (support) of the object, which…

Materials Science · Physics 2020-08-27 Vincent Favre-Nicolin , Steven Leake , Yuriy Chushkin

We propose to use intensity correlation microscopy in combination with structured illumination to image quantum emitters that exhibit antibunching with a spatial resolution reaching far beyond the Rayleigh limit. Combining intensity…

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

High-resolution diffusion tensor imaging (DTI) is beneficial for probing tissue microstructure in fine neuroanatomical structures, but long scan times and limited signal-to-noise ratio pose significant barriers to acquiring DTI at…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Qiyuan Tian , Ziyu Li , Qiuyun Fan , Chanon Ngamsombat , Yuxin Hu , Congyu Liao , Fuyixue Wang , Kawin Setsompop , Jonathan R. Polimeni , Berkin Bilgic , Susie Y. Huang

A miniaturized full-frequency encoded illumination (mini-FEI) chip is presented for high-throughput super-resolution imaging using the spatial frequency shift (SFS) effect. A tunable full SFS scheme is achieved through propagating and…

Coherent diffractive imaging (CDI) enables lensless imaging with experimental simplicity and a flexible field of view, yet its resolution is fundamentally constrained by the Abbe diffraction limit. To overcome this limitation, we introduce…

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

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