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3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or…

Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up. With modern instrumentation this limitation can be lifted significantly but some…

Data Analysis, Statistics and Probability · Physics 2018-05-23 Matthias Patting , Paja Reisch , Marcus Sackrow , Rhys Dowler , Marcelle Koenig , Michael Wahl

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

Efficient detection of single optical centers in solids is essential for quantum information processing, sensing, and single-photon generation applications. In this work, we use radio-frequency (RF) reflectometry to electrically detect the…

For active optical imaging, the use of single-photon detectors can greatly improve the detection sensitivity of the system. However, the traditional maximum-likelihood based imaging method needs a long acquisition time to capture clear…

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

A system for time-correlated single photon counting applications based on CMOS single photon avalanche diodes is presented. An instrument response function of 79 ps allows a fluorescence decay of high-affinity Ca2+ indicator Oregon Green…

Soft Condensed Matter · Physics 2008-03-24 M. Gersbach , D. L. Boiko , C. Niclass , C. Petersen , E. Charbon

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

Recently, there has been increased attention towards 3D imaging using single-pixel single-photon detection (also known as temporal data) due to its potential advantages in terms of cost and power efficiency. However, to eliminate the…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Tingqin Lai , Xiaolin Liang , Yi Zhu , Xinyi Wu , Lianye Liao , Xuelin Yuan , Ping Su , Shihai Sun

Photoluminesce (PL) spectroscopy offers excellent methods for mapping the PL decay on the nanosecond time scale. However, capturing maps of emission dynamics on the microsecond time scale can be highly time-consuming. We present a new…

Optics · Physics 2021-02-24 Jiri Junek , Karel Zidek

3D single-photon LiDAR imaging has an important role in many applications. However, full deployment of this modality will require the analysis of low signal to noise ratio target returns and a very high volume of data. This is particularly…

Image and Video Processing · Electrical Eng. & Systems 2023-07-19 Mohamed Amir Alaa Belmekki , Jonathan Leach , Rachael Tobin , Gerald S. Buller , Stephen Mclaughlin , Abderrahim Halimi

We demonstrate a photon-sensitive, three-dimensional camera by active near-infrared illumination and fast time-of-flight gating. It uses pico-second pump pulses to selectively up-convert the backscattered photons according to their…

Optics · Physics 2022-11-01 He Zhang , Santosh Kumar , Yong Meng Sua , Shenyu Zhu , Yu-Ping Huang

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Long-range active imaging has a variety of applications in remote sensing and target recognition. Single-photon LiDAR (light detection and ranging) offers single-photon sensitivity and picosecond timing resolution, which is desirable for…

Image and Video Processing · Electrical Eng. & Systems 2020-09-07 Zheng-Ping Li , Xin Huang , Yuan Cao , Bin Wang , Yu-Huai Li , Weijie Jin , Chao Yu , Jun Zhang , Qiang Zhang , Cheng-Zhi Peng , Feihu Xu , Jian-Wei Pan

We present a method for reconstructing 3D shape of arbitrary Lambertian objects based on measurements by miniature, energy-efficient, low-cost single-photon cameras. These cameras, operating as time resolved image sensors, illuminate the…

Computer Vision and Pattern Recognition · Computer Science 2024-04-02 Fangzhou Mu , Carter Sifferman , Sacha Jungerman , Yiquan Li , Mark Han , Michael Gleicher , Mohit Gupta , Yin Li

In order to discern aggregation in solutions, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the…

Biological Physics · Physics 2009-11-10 Hai-cang Ren , Noel L. Goddard , Gregoire Altan-Bonnet , Albert Libchaber

Time-of-flight three dimensional imaging is an important tool for many applications, such as object recognition and remote sensing. Unlike conventional imaging approach using pixelated detector array, single-pixel imaging based on projected…

We demonstrated a laser depth imaging system based on the time-correlated single-photon counting technique, which was incorporated with a low-jitter superconducting nanowire single-photon detector (SNSPD), operated at the wavelength of 1550…

Optics · Physics 2023-07-19 H. Zhou , Y. He , L. You , S. Chen , W. Zhang , J. Wu , Z. Wang , X. Xie

Frequency-filtered photon correlations have been proven to be extremely useful in grasping how the detection process alters photon statistics. Harnessing the spectral correlations also permits refinement of the emission and unraveling of…

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