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Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by…

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

Visualization in the virtual image formed by dielectric microparticles has been shown to enable the distinction of objects that remain indistinguishable under direct observation. We perform the resolution analysis based on a full…

Flexible microelectrode (FME) implantation into brain cortex is challenging due to the deformable fiber-like structure of FME probe and the interaction with critical bio-tissue. To ensure reliability and safety, the implantation process…

Computer Vision and Pattern Recognition · Computer Science 2025-10-13 Yitong Chen , Xinyao Xu , Ping Zhu , Xinyong Han , Fangbo Qin , Shan Yu

The theoretically observable limit of electron density distribution by single-crystal X-ray diffraction is discussed. When F_{orb} and {\delta}F are defined as, respectively, the partial structure factor for an orbital and the deviation of…

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

Creating arbitrary light patterns finds applications in various domains including lithography, beam shaping, metrology, sensing and imaging. We study the formation of high-contrast light patterns that are obtained by transmission through an…

Optics · Physics 2016-11-23 Ali Naqavi , Hans Peter Herzig , Markus Rossi

Optimal embedding in the three-dimensional space of exponentially growing squeezed surfaces, like plants leaves, or 2D colonies of exponentially reproducing cells, is considered in the framework of conformal approach. It is shown that the…

Soft Condensed Matter · Physics 2016-11-01 Sergei Nechaev , Kirill Polovnikov

There is a clear need for developments in characterisation techniques that provide detailed information about structure-function relationships in biology. Using electron microscopy to achieve high resolution while maintaining a broad field…

Quantitative Methods · Quantitative Biology 2023-09-12 Chen Huang , Judy S. Kim , Angus I. Kirkland

Microtomography is a powerful method of materials investigation. It enables to obtain physical properties of porous media non-destructively that is useful in studies. One of the application ways is a calculation of porosity, pore sizes,…

Computational Physics · Physics 2020-07-08 Maxim Grigoriev , Anvar Khafizov , Vladislav Kokhan , Viktor Asadchikov

Free-space optical information transfer through diffusive media is critical in many applications, such as biomedical devices and optical communication, but remains challenging due to random, unknown perturbations in the optical path. In…

Optics · Physics 2023-08-29 Yuhang Li , Tianyi Gan , Bijie Bai , Cagatay Isil , Mona Jarrahi , Aydogan Ozcan

Classification of an object behind a random and unknown scattering medium sets a challenging task for computational imaging and machine vision fields. Recent deep learning-based approaches demonstrated the classification of objects using…

Optics · Physics 2023-03-10 Yi Luo , Bijie Bai , Yuhang Li , Ege Cetintas , Aydogan Ozcan

The simple entropic method to statistical reconstructing of heterogeneous three-dimensional media from a single two-dimensional image is briefly reported. We apply the entropic descriptor quantifying spatial inhomogeneity that depends on…

Statistical Mechanics · Physics 2015-11-18 D. Frączek , W. Olchawa , R. Piasecki , R. Wiśniowski

Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information. However, the classical approach of…

Computer Vision and Pattern Recognition · Computer Science 2022-08-22 Fengjiao Ma , Rainer Kaufmann , Jaroslaw Sedzicki , Zoltán Cseresnyés , Christoph Dehio , Stephanie Hoeppener , Marc Thilo Figge , Rainer Heintzmann

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

It is generally assumed that a high spatial resolution of a microscope requires a large numerical aperture of the imaging lens or detector. In this study, the information limit of 15 pm is achieved in transmission electron microscopy using…

Materials Science · Physics 2024-01-08 Haozhi Sha , Jizhe Cui , Wenfeng Yang , Rong Yu

Surface topography dictates the deterministic functionality of diffraction by a surface. In order to maximize the efficiency with which a diffractive optical component, such as a grating or a diffractive lens, directs light into a chosen…

Structured materials like photonic crystals require for optimal use a high precision both on position and optical characteristics of the components which they are made of. Here, we present a simple tomographic algorithm, based on a specific…

Optics · Physics 2009-11-13 Jean-Phiilppe Groby , Dominique Lesselier

In developing medical interventions using untethered milli- and microrobots, ensuring safety and effectiveness relies on robust methods for detection, real-time tracking, and precise localization within the body. However, the inherent…

Robotics · Computer Science 2024-09-16 Husnu Halid Alabay , Tuan-Anh Le , Hakan Ceylan