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This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy. Our results show that by including a model of illumination within the first layers of a deep convolutional neural network, it is…

Image and Video Processing · Electrical Eng. & Systems 2020-04-23 Colin L. Cooke , Fanjie Kong , Amey Chaware , Kevin C. Zhou , Kanghyun Kim , Rong Xu , D. Michael Ando , Samuel J. Yang , Pavan Chandra Konda , Roarke Horstmeyer

Diffusion models (DMs) have become dominant in visual generation but suffer performance drop when tested on resolutions that differ from the training scale, whether lower or higher. In fact, the key challenge in generating variable-scale…

Computer Vision and Pattern Recognition · Computer Science 2025-11-25 Guohui Zhang , Jiangtong Tan , Linjiang Huang , Zhonghang Yuan , Mingde Yao , Jie Huang , Feng Zhao

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Light localization and intensity enhancement in a woodpile layer-by-layer photonic crystal, whose interlayer distance along the propagation direction is gradually varied, has been theoretically predicted and experimentally demonstrated. The…

Optics · Physics 2017-07-17 Zeki Hayran , Hamza Kurt , Kestutis Staliunas

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

In both light optics and electron optics, the amplitude of a wave scattered by an object is an observable that is usually recorded in the form of an intensity distribution in a real space image or a diffraction image. In contrast, retrieval…

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Diffusion models are a special type of generative model, capable of synthesising new data from a learnt distribution. We introduce DISPR, a diffusion-based model for solving the inverse problem of three-dimensional (3D) cell shape…

Computer Vision and Pattern Recognition · Computer Science 2023-03-15 Dominik J. E. Waibel , Ernst Röell , Bastian Rieck , Raja Giryes , Carsten Marr

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

Differential Dynamic Microscopy (DDM) analyzes traditional real-space microscope images to extract information on sample dynamics in a way akin to light scattering, by decomposing each image in a sequence into Fourier modes, and evaluating…

Soft Condensed Matter · Physics 2017-11-10 Fabio Giavazzi , Paolo Edera , Peter J. Lu , Roberto Cerbino

An extended interference pattern close to surface may result in both a transmissive or evanescent surface fields for large area manipulation of trapped particles. The affinity of differing particle sizes to a moving standing wave light…

The thin and flexible nature of optical fibres often makes them the ideal technology to view biological processes in-vivo, but current microendoscopic approaches are limited in spatial resolution. Here, we demonstrate a new route to high…

How microorganisms respond to and interact with their environment can vary significantly from individual to individual, which can have important microbiological and ecological implications. However, most microscopy techniques can only…

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

A simple method was developed to observe the interference patterns of the light reflected by the interfaces of thin liquid films. Employing a fluorescent microscope with epi-illumination, we collected the 2D patterns of interference fringes…

Optics · Physics 2010-06-14 V. Berejnov , D. Li

The dark entoptic Maxwell spot centroids seen through a blue filter, which coincide with the blue cone-free areas centered on the foveas, are shown to exhibit a structure. When observing through the green part of a blue-green exchange…

Biological Physics · Physics 2025-02-26 Albert Le Floch , Guy Ropars

We consider the inverse problem of denoising an image where each point (pixel) is an element of a target set, which we refer to as a target-valued image. The target sets considered are either (i) a closed convex set of Euclidean space or…

Numerical Analysis · Mathematics 2018-06-20 Braxton Osting , Dong Wang

This paper concerns electromagnetic 3D subsurface imaging in connection with sparsity of signal sources. We explored an imaging approach that can be implemented in situations that allow obtaining a large amount of data over a surface or a…

Instrumentation and Methods for Astrophysics · Physics 2016-08-26 Sampsa Pursiainen , Mikko Kaasalainen

Image retargeting, which resizes images to one with a prescribed aspect ratio by determining an optimal warping map, has gained substantial interest in imaging science. Despite significant advances, existing methods often fail to ensure…

Numerical Analysis · Mathematics 2025-10-16 Chengyang Liu , Michael K. Ng

The diffraction patterns of crystalline materials with local order contain sharp Bragg reflections as well as highly structured diffuse scattering. The instrumental requirements, experimental parameters and data processing techniques for…

Materials Science · Physics 2023-11-29 Romy Poppe , Joke Hadermann