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Optical microscopy has long been the standard method for diagnosis in cytopathology. Whole slide scanners can image and digitize large sample areas automatically, but are slow, expensive and therefore not widely available. Clinical…

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…

Quantitative Methods · Quantitative Biology 2012-02-03 Juliane Liepe , Federico Felizzi , Agata Pernus , Maria Hanulova

High-throughput screening using cell images is an efficient method for screening new candidates for pharmaceutical drugs. To complete the screening process, it is essential to have an efficient process for analyzing cell images. This paper…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Mizuki Fukasawa , Tomokazu Fukuda , Takuya Akashi

Tracking cells in time-lapse videos is an essential technique for monitoring cell population dynamics at a single-cell level. Current methods for cell tracking are developed on videos with mostly single, constant signals and do not detect…

Computer Vision and Pattern Recognition · Computer Science 2025-12-17 Florian Bürger , Martim Dias Gomes , Nica Gutu , Adrián E. Granada , Noémie Moreau , Katarzyna Bozek

Imaging flow cytometry shows significant potential for increasing our understanding of heterogeneous and complex life systems and is useful for biomedical applications. Ghost cytometry is a recently proposed approach for directly analyzing…

Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

Mitosis nuclei count is one of the important indicators for the pathological diagnosis of breast cancer. The manual annotation needs experienced pathologists, which is very time-consuming and inefficient. With the development of deep…

Computer Vision and Pattern Recognition · Computer Science 2022-12-29 Huadeng Wang , Zhipeng Liu , Rushi Lan , Zhenbing Liu , Xiaonan Luo , Xipeng Pan , Bingbing Li

3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…

Computer Vision and Pattern Recognition · Computer Science 2024-03-21 Kaito Shiku , Hiromitsu Shirai , Takeshi Ishihara , Ryoma Bise

Mitosis count is an important biomarker for prognosis of various cancers. At present, pathologists typically perform manual counting on a few selected regions of interest in breast whole-slide-images (WSIs) of patient biopsies. This task is…

Computer Vision and Pattern Recognition · Computer Science 2018-08-01 Saad Ullah Akram , Talha Qaiser , Simon Graham , Juho Kannala , Janne Heikkilä , Nasir Rajpoot

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…

As the frontline data for cancer diagnosis, microscopic pathology images are fundamental for providing patients with rapid and accurate treatment. However, despite their practical value, the deep learning community has largely overlooked…

Computer Vision and Pattern Recognition · Computer Science 2025-06-23 Sungrae Hong , Hyeongmin Park , Youngsin Ko , Sol Lee , Bryan Wong , Mun Yong Yi

Live-cell microscopy allows to go beyond measuring average features of cellular populations to observe, quantify and explain biological heterogeneity. Deep Learning-based instance segmentation and cell tracking form the gold standard…

Quantitative Methods · Quantitative Biology 2024-08-09 Richard D. Paul , Johannes Seiffarth , Hanno Scharr , Katharina Nöh

Pathology deals with the practice of discovering the reasons for disease by analyzing the body samples. The most used way in this field, is to use histology which is basically studying and viewing microscopic structures of cell and tissues.…

Image and Video Processing · Electrical Eng. & Systems 2021-11-08 Virender Ranga , Shivam Gupta , Priyansh Agrawal , Jyoti Meena

Quantitative analysis of the dynamics of tiny cellular and sub-cellular structures, known as particles, in time-lapse cell microscopy sequences requires the development of a reliable multi-target tracking method capable of tracking numerous…

Computer Vision and Pattern Recognition · Computer Science 2015-07-24 Seyed Hamid Rezatofighi , Stephen Gould , Ba Tuong Vo , Ba-Ngu Vo , Katarina Mele , Richard Hartley

The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…

Optics · Physics 2022-09-07 Lukas Wesemann , Jon Rickett , Timothy J. Davis , Ann Roberts

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Mitotic figure detection remains a challenging task in computational pathology due to domain variability and morphological complexity. This paper describes our participation in the MIDOG 2025 challenge, focusing on robust mitotic figure…

Image and Video Processing · Electrical Eng. & Systems 2025-09-04 Euiseop Song , Jaeyoung Park , Jaewoo Park

Most tumor grading systems for human as for veterinary histopathology are based upon the absolute count of mitotic figures in a certain reference area of a histology slide. Since time for prognostication is limited in a diagnostic setting,…

Computer Vision and Pattern Recognition · Computer Science 2018-10-23 Marc Aubreville , Christof A. Bertram , Robert Klopfleisch , Andreas Maier
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