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Manual count of mitotic figures, which is determined in the tumor region with the highest mitotic activity, is a key parameter of most tumor grading schemes. It can be, however, strongly dependent on the area selection due to uneven mitotic…

Imaging assays of cellular function, especially those using fluorescent stains, are ubiquitous in the biological and medical sciences. Despite advances in computer vision, such images are often analyzed using only manual or rudimentary…

Computer Vision and Pattern Recognition · Computer Science 2017-06-06 Lena R. Bartell , Lawrence J. Bonassar , Itai Cohen

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Our work targets automated analysis to quantify the growth dynamics of a population of bacilliform bacteria. We propose an innovative approach to frame-sequence tracking of deformable-cell motion by the automated minimization of a new,…

Computer Vision and Pattern Recognition · Computer Science 2022-07-12 Sorena Sarmadi , James J. Winkle , Razan N. Alnahhas , Matthew R. Bennett , Krešimir Josić , Andreas Mang , Robert Azencott

In this work we propose an approach to select the classification method and features, based on the state-of-the-art, with best performance for diagnostic support through peripheral blood smear images of red blood cells. In our case we used…

Machine Learning · Computer Science 2020-10-12 Nataša Petrović , Gabriel Moyà-Alcover , Antoni Jaume-i-Capó , Manuel González-Hidalgo

In this paper, we propose a hybrid approach for multi-object microbial cell segmentation. The approach combines an ML-based detection with a geometry-aware variational-based segmentation using B-splines that are parametrized based on a…

Computer Vision and Pattern Recognition · Computer Science 2022-05-04 Karina Ruzaeva , Katharina Nöh , Benjamin Berkels

Fluorescence microscopy is a major driver of scientific progress in the life sciences. Although high-end confocal microscopes are capable of filtering out-of-focus light, cheaper and more accessible microscopy modalities, such as widefield…

Image and Video Processing · Electrical Eng. & Systems 2026-04-08 Anirban Ray , Ashesh Ashesh , Florian Jug

A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…

Image and Video Processing · Electrical Eng. & Systems 2019-09-10 Shital Devinder , Ashish Lal , Tathagato Rai Dastidar , Satish Kumar Dubey

Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith…

Optics · Physics 2014-05-14 Shalin B. Mehta , Rudolf Oldenbourg

Most recently, the pathology diagnosis of cancer is shifting to integrating molecular makers with histology features. It is a urgent need for digital pathology methods to effectively integrate molecular markers with histology, which could…

Image and Video Processing · Electrical Eng. & Systems 2023-06-28 Xiaofei Wang , Stephen Price , Chao Li

Breast Cancer is the most prevalent cancer in the world. The World Health Organization reports that the disease still affects a significant portion of the developing world citing increased mortality rates in the majority of low to middle…

Computer Vision and Pattern Recognition · Computer Science 2021-08-23 Walt Williams , Jimmy Hall

Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Pingli Ma , Chen Li , Md Mamunur Rahaman , Yudong Yao , Jiawei Zhang , Shuojia Zou , Xin Zhao , Marcin Grzegorzek

Imaging airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using…

This work discusses preliminary work aimed at simulating and visualizing the growth process of a tiny structure inside the cell---the microtubule. Difficulty of recording the process lies in the fact that the tissue preparation method for…

Computer Vision and Pattern Recognition · Computer Science 2011-05-31 Feiyang Yu , Ard Oerlemans , Erwin M. Bakker

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Deviation of blood flow from an optimal range is known to be associated with the initiation and progression of vascular pathologies. Important open questions remain about how the abnormal flow drives specific wall changes in pathologies…

At advanced process nodes, lithography weakpoints can exist in physical layouts of integrated circuit designs even if the layouts pass design rule checking (DRC). Existence of lithography weakpoints in a physical layout can cause…

Other Computer Science · Computer Science 2018-10-04 I-Lun Tseng , Yongfu Li , Valerio Perez , Vikas Tripathi , Zhao Chuan Lee , Jonathan Yoong Seang Ong

Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi
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