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Automated detection and classification of mitotic figures especially distinguishing atypical from normal remain critical challenges in computational pathology. We present MitoDetect++, a unified deep learning pipeline designed for the MIDOG…

Image and Video Processing · Electrical Eng. & Systems 2025-09-08 Esha Sadia Nasir , Jiaqi Lv , Mostafa Jahanifar , Shan E Ahmed Raza

This paper introduces a novel framework for the automated tracking of cells, with a particular focus on the challenging situation of phase contrast microscopic videos. Our framework is based on a topology preserving variational segmentation…

Quantitative Methods · Quantitative Biology 2012-03-28 Michael Moeller , Martin Burger , Peter Dieterich , Albrecht Schwab

Atypical mitotic figures (AMFs) are important histopathological markers yet remain challenging to identify consistently, particularly under domain shift stemming from scanner, stain, and acquisition differences. We present a simple…

Computer Vision and Pattern Recognition · Computer Science 2025-08-29 Kaustubh Atey , Sameer Anand Jha , Gouranga Bala , Amit Sethi

Accurate and reliable nuclei identification is an essential part of quantification in microscopy. A range of mathematical and machine learning approaches are used but all methods have limitations. Such limitations include sensitivity to…

Quantitative Methods · Quantitative Biology 2019-04-11 Carl J. Nelson , Philip T. G. Jackson , Boguslaw Obara

Cell tracking and segmentation assist biologists in extracting insights from large-scale microscopy time-lapse data. Driven by local accuracy metrics, current tracking approaches often suffer from a lack of long-term consistency and the…

Computer Vision and Pattern Recognition · Computer Science 2025-06-30 Timo Kaiser , Maximilian Schier , Bodo Rosenhahn

The automated segmentation and tracking of macrophages during their migration are challenging tasks due to their dynamically changing shapes and motions. This paper proposes a new algorithm to achieve automatic cell tracking in time-lapse…

Image and Video Processing · Electrical Eng. & Systems 2023-01-03 Seol Ah Park , Tamara Sipka , Zuzana Kriva , George Lutfalla , Mai Nguyen-Chi , Karol Mikula

In this paper, we propose a new model to segment cells in phase contrast microscopy images. Cell images collected from the similar scenario share a similar background. Inspired by this, we separate cells from the background in images by…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Lin Zhang

Analyzing microscopy images to extract biological object properties (e.g., their morphological organization, temporal dynamics, and population density) is fundamental to various biomedical research. Yet conducting this manually is costly…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Xiaofei Hui , Haoxuan Qu , Hossein Rahmani , Shuohong Wang , Jeff W. Lichtman , Jun Liu

Histopathological prognostication of neoplasia including most tumor grading systems are based upon a number of criteria. Probably the most important is the number of mitotic figures which are most commonly determined as the mitotic count…

Computer Vision and Pattern Recognition · Computer Science 2020-01-06 Marc Aubreville , Christof A. Bertram , Robert Klopfleisch , Andreas Maier

Mitotic figures represent a key histoprognostic feature in tumor pathology, providing crucial insights into tumor aggressiveness and proliferation. However, their identification remains challenging, subject to significant inter-observer…

Image and Video Processing · Electrical Eng. & Systems 2025-10-21 Raphaël Bourgade , Guillaume Balezo , Hana Feki , Lily Monier , Matthieu Blons , Alice Blondel , Delphine Loussouarn , Anne Vincent-Salomon , Thomas Walter

Mitotic figure detection in histopathology images remains challenging due to significant domain shifts across different scanners, staining protocols, and tissue types. This paper presents our approach for the MIDOG 2025 challenge Track 1,…

The simulation of the metabolism in mammalian cells becomes a severe problem if spatial distributions must be taken into account. Especially the cytoplasm has a very complex geometric structure which cannot be handled by standard…

Numerical Analysis · Mathematics 2010-03-01 Michael Hanke , Marry-Chriz Cabauatan-Villanueva

We present a novel approach which extends the existing Fully Convolutional One-Stage Object Detector (FCOS) for mitotic figure detection. Our composite model adds a Feedback Attention Ladder CNN (FAL-CNN) model for classification of normal…

Image and Video Processing · Electrical Eng. & Systems 2025-09-22 Andrew Broad , Jason Keighley , Lucy Godson , Alex Wright

We here propose an automated pipeline for the microscopy image-based characterization of catalytically active inclusion bodies (CatIBs), which includes a fully automatic experimental high-throughput workflow combined with a hybrid approach…

Quantitative Methods · Quantitative Biology 2022-10-03 Karina Ruzaeva , Kira Küsters , Wolfgang Wiechert , Benjamin Berkels , Marco Oldiges , Katharina Nöh

The vast majority of the microscopy methods currently available to study biological samples require staining prior to imaging. Nevertheless, the ability to reveal specific cell structures or organelles in a label-free manner remains…

Biological Physics · Physics 2020-10-06 R. Desapogu , G. Le Marchand , R. Smith , P. Ray , E. Gillier , S. Dutertre , M. Alouini , M. Tramier , S. Huet , J. Fade

Combining experiments with artificial intelligence algorithms, we propose a new machine learning based approach to extract the cellular force distributions from the microscope images. The full process can be divided into three steps. First,…

We present cytometric classification of live healthy and cancer cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor…

Quantitative Methods · Quantitative Biology 2020-01-23 Darina Roitshtain , Lauren Wolbromsky , Evgeny Bal , Hayit Greenspan , Lisa L. Satterwhite , Natan T. Shaked

We present a new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis. Cell counting is an important step in cell analysis. Typically, domain experts manually…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Abdurahman Ali Mohammed , Catherine Fonder , Donald S. Sakaguchi , Wallapak Tavanapong , Surya K. Mallapragada , Azeez Idris

This paper presents a new method for automatic quantification of ellipse-like cells in images, an important and challenging problem that has been studied by the computer vision community. The proposed method can be described by two main…

Computer Vision and Pattern Recognition · Computer Science 2012-01-17 Wesley Nunes Gonçalves , Odemir Martinez Bruno

Fluorescence microscopy is a widely used method among cell biologists for studying the localization and co-localization of fluorescent protein. For microbial cell biologists, these studies often include tedious and time-consuming manual…

Computer Vision and Pattern Recognition · Computer Science 2020-09-29 Jonathan Reiner , Guy Azran , Gal Hyams