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This paper considers homography estimation in a Bayesian filtering framework using rate gyro and camera measurements. The use of rate gyro measurements facilitates a more reliable estimate of homography in the presence of occlusions, while…

Robotics · Computer Science 2023-10-17 Arturo Del Castillo Bernal , Philippe Decoste , James Richard Forbes

We present a formalism to discern the effects of fluctuations of the spacetime metric on electromagnetic radiation. The formalism works via the measurement of electromagnetic field correlations, while allowing a clear assessment of the…

General Relativity and Quantum Cosmology · Physics 2024-04-09 B. Sharmila , Sander M. Vermeulen , Animesh Datta

Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…

Instrumentation and Detectors · Physics 2018-10-12 Matthias Eibl , Sebastian Karpf , Hubertus Hakert , Daniel Weng , Robert Huber

Here we report on the experimental details of a study on the influence of the photonic environment on the emission of a FRET system. We modified the local density of optical states (LDOS) by placing the FRET system at precisely defined…

Chemical Physics · Physics 2012-11-26 Niels Zijlstra , Allard P. Mosk , Willem L. Vos , Vinod Subramaniam , Christian Blum

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

Optics · Physics 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Fluorescence microscopy is of vital importance for understanding biological function. However most fluorescence experiments are only qualitative inasmuch as the absolute number of fluorescent particles can often not be determined.…

A long-held vision has been to realize diffraction-limited optical aperture synthesis over kilometer baselines. This will enable imaging of stellar surfaces and their environments, and reveal interacting gas flows in binary systems. An…

Instrumentation and Methods for Astrophysics · Physics 2015-08-12 Dainis Dravins , Tiphaine Lagadec , Paul D. Nuñez

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at sub-micron…

Optics · Physics 2021-04-08 Yi Zhang , Haonan Zong , Cheng Zong , Yuying Tan , Meng Zhang , Yuewei Zhan , Ji-Xin Cheng

Applications of Fluorescence Resonance Energy Transfer (FRET) in biology have expanded tremendously in the last 25 years. This technique has become a staple technique in many biological and biophysical fields. FRET based chemical sensors…

Biological Physics · Physics 2020-05-05 Ripa Paul , Sudip Suklabaidya , Syed Arshad Hussain

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

We present a sensing scheme for estimating the frequency difference of two non-entangled photons. The technique consists of time-resolving sampling measurements at the output of a beam splitter. With this protocol, the frequency shift…

Quantum Physics · Physics 2025-12-10 Luca Maggio , Danilo Triggiani , Paolo Facchi , Vincenzo Tamma

Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approaches, however, presents challenges for low-light…

Fluorescence molecular tomography (FMT) is a promising modality for non-invasive imaging of internal fluorescence agents in biological tissues especially in small animal models, with applications in diagnosis, therapy, and drug design. In…

Optics · Physics 2023-08-09 Wenxiang Cong , Ge Wang

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

We review the continuous monitoring of a qubit through its spontaneous emission, at an introductory level. Contemporary experiments have been able to collect the fluorescence of an artificial atom in a cavity and transmission line, and then…

Quantum Physics · Physics 2020-09-28 Philippe Lewalle , Sreenath K. Manikandan , Cyril Elouard , Andrew N. Jordan

Atomic Force Microscopy - Infrared (AFM-IR) has emerged as a useful technique for measuring absorption spectra with spatial resolution better than the optical diffraction limit. The technique relies on the movement of a probe for atomic…

Applied Physics · Physics 2024-01-17 Luca Quaroni

Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit…

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…