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Photometric instruments operating at far infrared to millimetre wavelengths often have broad spectral passbands (central wavelength/bandwidth ~ 3 or less), especially those operating in space. A broad passband can result in significant…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in…

Fourier Ptychographic Microscopy (FPM) is a recent technique to overcome the diffraction limit of a low numerical aperture (NA) objective lens by algorithmic post-processing of several lower resolved images. It can increase the…

Atom probe tomography (APT) helps elucidate the link between the nanoscale chemical variations and physical properties, but it has limited structural resolution. Field ion microscopy (FIM), a predecessor technique to APT, is capable of…

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

Monitoring the evolution of the anthropogenic light emissions is a priority task in light pollution research. Among the complementary approaches that can be adopted to achieve this goal stand out those based on measuring the direct radiance…

Instrumentation and Methods for Astrophysics · Physics 2024-10-31 Salvador Bará

We present a new method that combines fluorescence correlation spectroscopy (FCS) on the microsecond time scale with fluorescence antibunching measurements on the nanosecond time scale for measuring photophysical rate constants of…

Chemical Physics · Physics 2022-03-28 Damir Sakhapov , Ingo Gregor , Narain Karedla , Jörg Enderlein

Measurement of interferometric parameters values is affected by phase disturbance due especially to atmospheric turbulences. Algorithms of fringe sensing, aimed at fringe parameters identification, are based on interferometric models that…

Instrumentation and Methods for Astrophysics · Physics 2009-08-27 Bonino Donata

A Michelson interferometer with noise reduction to sub-shot noise levels is proposed and realized. Multiple measurements of a single signal beam are taken and the quantum property of light plays an essential role in the principle underlying…

Optics · Physics 2012-06-19 Takahisa Mitsui , Kenichiro Aoki

An interferometric imaging technique has been proposed to instantly measure the diameter of individual spherical dust particles suspended in a gas discharge plasma. The technique is based on the defocused image analysis of both spherical…

Plasma Physics · Physics 2016-04-06 M. Chaudhuri , V. Nosenko , H. M. Thomas

Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…

Optics · Physics 2019-05-22 Oumeng Zhang , Matthew D. Lew

Intensity, wavevector, phase, and polarization are the most important parameters of any light beam. Understanding the wavevector distribution has emerged as a very important problem in recent days, especially at nanoscale. It provides…

Optics · Physics 2020-01-22 Adarsh B. Vasista , Deepak K. Sharma , G V Pavan Kumar

The paper presents an alternative way to classical stereocorrelation. First, 2D image processing of random patterns is described. Sub-pixel displacements are determined using phase analysis. Then distortion evaluation is presented. The…

Classical Physics · Physics 2013-11-18 Jérôme Molimard , Gaetan Boyer , Hassan Zahouani

Context. Filamentary structures appear to be ubiquitous in the interstellar medium. Being able to detect and characterize them is the first step toward understanding their origin, their evolution, and their role in the Galactic cycle of…

Astrophysics of Galaxies · Physics 2022-12-14 J. -S. Carrière , L. Montier , K. Ferrière , I. Ristorcelli

We study theoretically the accuracy of the method based on the Fourier property of lenses that is commonly used for the far field measurement. We consider a simple optical setup in which the far-field intensity pattern of a light beam…

Optics · Physics 2013-07-19 Pierre Suret , Stephane Randoux

We show how methods from Hamiltonian Floer theory can be used to establish lower bounds for the number of different time-periodic measures of time-periodic Hamiltonian systems with diffusion. After proving the existence of closed random…

Symplectic Geometry · Mathematics 2023-08-10 Oliver Fabert
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