Related papers: Force spectroscopy in studying infection
Optical tweezers exploit light--matter interactions to trap particles ranging from single atoms to micrometer-sized eukaryotic cells. For this reason, optical tweezers are a ubiquitous tool in physics, biology, and nanotechnology. Recently,…
This review article provides an overview of structurally oriented experimental datasets that can be used to benchmark protein force fields, focusing on data generated by nuclear magnetic resonance (NMR) spectroscopy and room temperature…
Since its invention by Arthur Ashkin and colleagues at Bell Labs in the 1970s, optical micromanipulation, also known as optical tweezers or laser tweezers, has evolved remarkably to become one of the most convenient and versatile tools for…
X-ray spectroscopy is a powerful technique for the analysis of the energy distribution of X-rays from astrophysical sources. It allows for the study of the properties, composition, and physical processes taking place at the site of…
Spatial transcriptomics methods capture cellular measurements such as gene expression and cell types at specific locations in a cell, helping provide a localized picture of tissue health. Traditional visualization techniques superimpose the…
The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…
The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence…
The speckle statistics of optical coherence tomography images of biological tissue have been studied using several historical probability density functions. A recent hypothesis implies that underlying power-law distributions in the medium…
Introduced more than fifty years ago, dynamic light scattering is routinely used to determine the size distribution of colloidal suspensions, as well as of macromolecules in solution, such as proteins, nucleic acids, and their complexes.…
Achieving a comprehensive understanding of the behaviour of proteins is greatly facilitated by the knowledge of their structures, thermodynamics and dynamics. All this information can be provided in an effective manner in terms of…
Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…
Many polarisation techniques have been harnessed for decades in biological and clinical research, each based upon measurement of the vectorial properties of light or the vectorial transformations imposed on light by objects. Various…
Objective: Survival rate of oral squamous cell carcinoma (OSCC) patients is very poor and can be improved using highly sensitive, specific and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and useful…
We describe a novel application of atomic force microscopy (AFM) to directly visualize cytoskeletal fibers in human foreskin epithelial cells. The nonionic detergent Triton X-100 in a low concentration was used to remove the membrane,…
Dynamic speckle imaging, typically used in laser-illuminated surface diagnostics, has proven valuable for assessing biological activity. In this work, we demonstrate its feasibility in an endoscopic context using a disposable bronchoscope.…
New combinations of existing antibiotics are being investigated to combat bacterial resilience. This requires detection technologies with reasonable cost, accuracy, resolution, and throughput. Here, we present a multi -drug screening…
To illustrate the power of the biophysical approach in solving important problems in life science, I present here one of our current research projects as an example. We have developed special biophotonic techniques to study the dynamic…
Fourier-transform infrared spectroscopy (FTIR) is a powerful analytical method for not only the chemical identification of solid, liquid, and gas species, but also the quantification of their concentration. However, the chemical…
How local stresses propagate through polymeric fluids, and, more generally, how macromolecular dynamics give rise to viscoelasticity are open questions vital to wide-ranging scientific and industrial fields. Here, to unambiguously connect…
The structure of proteins is essential for its function. The determination of protein structures is possible by experimental or predicted by computational methods, but also a combination of both approaches is possible. Here, first an…