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Extensions and improvements of empirical force fields are discussed in view of applications to computational vibrational spectroscopy and reactive molecular dynamics simulations. Particular focus is on quantitative studies which make…

Chemical Physics · Physics 2020-07-08 D. Koner , M. S. Salehi , P. Mondal , M. Meuwly

Emulsions are ubiquitous in everyday life and find applications in various industries. Optical tweezers (OTs) have emerged as the preferred method for studying emulsion dynamics. In this review, we first introduce the theory of optical…

Proteins are the active working horses in our body. These biomolecules perform all vital cellular functions from DNA replication and general biosynthesis to metabolic signaling and environmental sensing. While static 3D structures are now…

Quantitative Methods · Quantitative Biology 2020-10-16 Sonja Schmid , Cees Dekker

Since the pioneering work of Arthur Ashkin, optical tweezers have become an indispensable tool for contactless manipulation of micro- and nanoparticles. Nowadays optical tweezers are employed in a myriad of applications demonstrating the…

Acoustics provides a powerful modality with which to 'see' the mechanical properties of a wide range of elastic materials. It is particularly adept at probing soft materials where excellent contrast and propagation distance can be achieved.…

Soft Condensed Matter · Physics 2009-05-01 N. G. Parker , M. J. W. Povey

Motivation: Biomarker discovery from high-dimensional data is a crucial problem with enormous applications in biology and medicine. It is also extremely challenging from a statistical viewpoint, but surprisingly few studies have…

Quantitative Methods · Quantitative Biology 2012-09-17 Anne-Claire Haury , Pierre Gestraud , Jean-Philippe Vert

Microrheology is a branch of rheology having the same principles as conventional bulk rheology, but working on micron length scales and micro-litre volumes. Optical tweezers have been successfully used with Newtonian fluids for rheological…

Photo-induced magnetic force between nanostructures, at optical frequencies, is investigated theoretically. Till now optical magnetic effects are not used in scanning probe microscopy because of the vanishing natural magnetism with…

Tapping mode atomic force microscopy is a standard technique for inspection and analysis at the nanometer scale. The understanding of the non-linear dynamics of the system due to the tip sample interaction is an important prerequisite for a…

Instrumentation and Detectors · Physics 2007-05-23 Robert W. Stark

Red blood cells possess unique biomechanical ability to squeeze through capillaries smaller than their size to enable gas and ion exchange. A key signature of their active biomechanics is the out-of-equilibrium fluctuation of the plasma…

An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Ryoichi Horisaki , Takuro Ideguchi

Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we describe how these methods can be extended to larger eukaryotic,…

Subcellular Processes · Quantitative Biology 2016-06-09 Adam J. M. Wollman , Mark C. Leake

Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of…

Soft Condensed Matter · Physics 2017-06-21 Luca Puricelli , Massimiliano Galluzzi , Carsten Schulte , Alessandro Podestà , Paolo Milani

Fourier transform spectroscopy (FTS) has been widely used as an analytical tool for many applications in science and engineering. In this paper, we describe the operation principle and practical implementation of an FTS prototype. First,…

Instrumentation and Detectors · Physics 2023-02-02 Sohrab Sheikh-Sofla , Mohammad Neshat

Atomic Force Microscopy - Infrared (AFM-IR) has emerged as a useful technique for measuring absorption spectra with spatial resolution better than the optical diffraction limit. The technique relies on the movement of a probe for atomic…

Applied Physics · Physics 2024-01-17 Luca Quaroni

Critical Casimir forces emerge between objects, such as colloidal particles, whenever their surfaces spatially confine the fluctuations of the order parameter of a critical liquid used as a solvent. These forces act at short but…

Soft Condensed Matter · Physics 2020-08-05 Agnese Callegari , Alessandro Magazzù , Andrea Gambassi , Giovanni Volpe

The native structures of proteins, except for notable exceptions of intrinsically disordered proteins, in general take their most stable conformation in the physiological condition to maintain their structural framework so that their…

Biomolecules · Quantitative Biology 2021-10-26 Lyman Monroe , Daisuke Kihara

Optical tweezers are widely used as a highly sensitive tool to measure forces on micron-scale particles. One such application is the measurement of the electric charge of a particle, which can be done with high precision in liquids, air, or…

Atomic force microscopy (AFM) is an essential nanoinstrument technique for several applications such as cell biology and nanoelectronics metrology and inspection. The need for statistically significant sample sizes means that data…

Instrumentation and Detectors · Physics 2017-04-05 H. Sadeghian , R. Herfst , B. Dekker , J. Winters , T. Bijnagte , R. Rijnbeek

Amplitude-modulation atomic force microscopy enables observation of fragile molecules at the nanometer scale. To shorten measurement times and capture dynamic molecules, increasing the frame rate is essential. Traditionally, maximum frame…

Applied Physics · Physics 2024-11-26 Kenichi Umeda , Noriyuki Kodera