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Sub-nm resolution images can be achieved by Atomic Force Microscopy (AFM) on samples that are deposited on hard substrates. However, it is still extremely challenging to image soft interfaces, such as biological membranes, due to the…

Optical tweezers have found widespread application in many fields, from physics to biology. Here, we explain in detail how optical forces and torques can be described within the geometrical optics approximation and we show that this…

Optics · Physics 2015-06-18 Agnese Callegari , Mite Mijalkov , A. Burak Gököz , Giovanni Volpe

In the present work, we examine the relevance and proper interpretation of broadband-dielectric and THz-spectroscopy data for the investigation of various types of biological matter. We provide an overview of the rich variety of different…

Soft Condensed Matter · Physics 2025-11-13 Peter Lunkenheimer , Sebastian Emmert , Martin Wolf , Alois Loidl

Precise manipulation of single molecules has already led to remarkable insights in physics, chemistry, biology and medicine. However, widespread adoption of single-molecule techniques has been impeded by equipment cost and the laborious…

Biological Physics · Physics 2015-05-14 Ken Halvorsen , Wesley P. Wong

Microorganisms are ubiquitous in nature, and microbial activities are closely intertwined with the entire life cycle system and human life. Developing novel technologies for the detection, characterization and manipulation of microorganisms…

Optics · Physics 2025-10-17 Ding Cao , Guangyou Fang , Xuequan Chen

Knowledge of surface forces is the key to understanding a large number of processes in fields ranging from physics to material science and biology. The most common method to study surfaces is dynamic atomic force microscopy (AFM). Dynamic…

Mesoscale and Nanoscale Physics · Physics 2013-02-06 Daniel Platz , Daniel Forchheimer , Erik A. Tholen , David B. Haviland

Optical tweezers are powerful tools for high resolution study of surface properties. Such experiments are traditionally performed by studying the active or the brownian fluctuation of trapped particles in the X, Y, Z direction. Here we find…

Optics · Physics 2018-11-13 Rahul Vaipully , Dhanush Bhatt , Anand Dev Ranjan , Basudev Roy

Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofibre and optical tweezers…

Polarization dynamics in ferroelectric materials are explored via the automated experiment in Piezoresponse Force Spectroscopy. A Bayesian Optimization framework for imaging is developed and its performance for a variety of acquisition and…

Disordered Systems and Neural Networks · Physics 2021-06-24 Rama K. Vasudevan , Kyle Kelley , Jacob Hinkle , Hiroshi Funakubo , Stephen Jesse , Sergei V. Kalinin , Maxim Ziatdinov

Atomic Force Microscopy (AFM) has become established as a powerful and a versatile tool for investigating local mechanical properties. In addition, it has been made possible to take advantage of the AFM tip-sample interaction, to perturb,…

The use of tapered optical fibers, i.e., optical nanofibers, for spectroscopy and the detection of small numbers of particles, such as neutral atoms or molecules, has been gaining ground in recent years. In this review, we briefly introduce…

Material indentation studies, in which a probe is brought into controlled physical contact with an experimental sample, have long been a primary means by which scientists characterize the mechanical properties of materials. More recently,…

Applications · Statistics 2011-10-03 Daniel Rudoy , Shelten G. Yuen , Robert D. Howe , Patrick J. Wolfe

Single-molecule stretching experiments on DNA, RNA, and other biological macromolecules opened up the possibility of an impressive progress in many fields of Life and Medical sciences. The reliability of such experiments may be crucially…

Soft Condensed Matter · Physics 2019-03-22 G. Florio , G Puglisi

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

The possibility of detecting mutations in a DNA from force measurements (as a first step towards sequence analysis) is discussed theoretically based on exact calculations. The force signal is associated with the domain wall separating the…

Statistical Mechanics · Physics 2009-11-07 Somendra M. Bhattacharjee , D. Marenduzzo

Atomic force microscopy (AFM) with molecule-functionalized tips has emerged as the primary experimental technique for probing the atomic structure of organic molecules on surfaces. Most experiments have been limited to nearly planar…

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofiber and optical tweezers…

Comprehensive characterization of nanomaterials for medical applications is a challenging and complex task due to the multitude of parameters which need to be taken into consideration in a broad range of conditions. Routine methods such as…

Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Sachin Shah , Sakshum Kulshrestha , Christopher A. Metzler
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