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In the field of connectomics, neuroscientists seek to identify cortical connectivity comprehensively. Neuronal boundary detection from the Electron Microscopy (EM) images is often done to assist the automatic reconstruction of neuronal…

Computer Vision and Pattern Recognition · Computer Science 2017-08-01 Wei Shen , Bin Wang , Yuan Jiang , Yan Wang , Alan Yuille

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

Computer Vision and Pattern Recognition · Computer Science 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

We present PyTorch Connectomics (PyTC), an open-source deep-learning framework for the semantic and instance segmentation of volumetric microscopy images, built upon PyTorch. We demonstrate the effectiveness of PyTC in the field of…

Image and Video Processing · Electrical Eng. & Systems 2021-12-14 Zudi Lin , Donglai Wei , Jeff Lichtman , Hanspeter Pfister

Electron Microscopy (EM) image (or volume) segmentation has become significantly important in recent years as an instrument for connectomics. This paper proposes a novel agglomerative framework for EM segmentation. In particular, given an…

Computer Vision and Pattern Recognition · Computer Science 2015-06-09 Toufiq Parag , Anirban Chakraborty , Stephen Plaza , Lou Scheffer

The alignment of serial-section electron microscopy (ssEM) images is critical for efforts in neuroscience that seek to reconstruct neuronal circuits. However, each ssEM plane contains densely packed structures that vary from one section to…

Computer Vision and Pattern Recognition · Computer Science 2017-12-06 Inwan Yoo , David G. C. Hildebrand , Willie F. Tobin , Wei-Chung Allen Lee , Won-Ki Jeong

Morphology based analysis of cell types has been an area of great interest to the neuroscience community for several decades. Recently, high resolution electron microscopy (EM) datasets of the mouse brain have opened up opportunities for…

Neuron segmentation from electron microscopy (EM) volumes is crucial for understanding brain circuits, yet the complex neuronal structures in high-resolution EM images present significant challenges. EM data exhibits unique characteristics…

Computer Vision and Pattern Recognition · Computer Science 2025-08-26 Yinda Chen , Haoyuan Shi , Xiaoyu Liu , Te Shi , Ruobing Zhang , Dong Liu , Zhiwei Xiong , Feng Wu

The field of connectomics faces unprecedented "big data" challenges. To reconstruct neuronal connectivity, automated pixel-level segmentation is required for petabytes of streaming electron microscopy data. Existing algorithms provide…

Mapping the connectivity of neurons in the brain (i.e., connectomics) is a challenging problem due to both the number of connections in even the smallest organisms and the nanometer resolution required to resolve them. Because of this,…

Neurons and Cognition · Quantitative Biology 2014-09-08 Ting Zhao , Stephen M Plaza

Producing connectomes from electron microscopy (EM) images has historically required a great deal of human proofreading effort. This manual annotation cost is the current bottleneck in scaling EM connectomics, for example, in making larger…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Gary B Huang , William M Katz , Stuart Berg , Louis Scheffer

Reconstructing the intricate local morphology of neurons and their long-range projecting axons can address many connectivity related questions in neuroscience. The main bottleneck in connectomics pipelines is correcting topological errors,…

Computer Vision and Pattern Recognition · Computer Science 2025-03-05 Anna Grim , Jayaram Chandrashekar , Uygar Sumbul

Segmentation of cellular structures in electron microscopy (EM) images is fundamental to analyzing the morphology of neurons and glial cells in the healthy and diseased brain tissue. Current neuronal segmentation applications are based on…

Computer Vision and Pattern Recognition · Computer Science 2024-11-06 Zewen Zhuo , Ilya Belevich , Ville Leinonen , Eija Jokitalo , Tarja Malm , Alejandra Sierra , Jussi Tohka

Despite the progress in deep learning networks, efficient learning at the edge (enabling adaptable, low-complexity machine learning solutions) remains a critical need for defense and commercial applications. We envision a pipeline to…

Mammalian whole-brain connectomes are a foundational ingredient for holistic understanding of brains. Indeed, imaging connectomes at sufficient resolution to densely reconstruct cellular morphology and synapses represents a longstanding…

Neurons and Cognition · Quantitative Biology 2025-02-03 Logan Thrasher Collins , Todd Huffman , Randal Koene

Large electron microscopy image datasets for connectomics are typically composed of thousands to millions of partially overlapping two-dimensional images (tiles), which must be registered into a coherent volume prior to further analysis. A…

Computer Vision and Pattern Recognition · Computer Science 2018-04-27 Khaled Khairy , Gennady Denisov , Stephan Saalfeld

We present a method combining affinity prediction with region agglomeration, which improves significantly upon the state of the art of neuron segmentation from electron microscopy (EM) in accuracy and scalability. Our method consists of a…

Computer Vision and Pattern Recognition · Computer Science 2020-07-29 Jan Funke , Fabian David Tschopp , William Grisaitis , Arlo Sheridan , Chandan Singh , Stephan Saalfeld , Srinivas C. Turaga

Graphs are quickly emerging as a leading abstraction for the representation of data. One important application domain originates from an emerging discipline called "connectomics". Connectomics studies the brain as a graph; vertices…

Recent successes in deep learning have started to impact neuroscience. Of particular significance are claims that current segmentation algorithms achieve "super-human" accuracy in an area known as connectomics. However, as we will show,…

Computer Vision and Pattern Recognition · Computer Science 2020-01-20 Drew Linsley , Junkyung Kim , David Berson , Thomas Serre

This paper explores the potential of brain-computer interfaces in segmenting objects from images. Our approach is centered around designing an effective method for displaying the image parts to the users such that they generate measurable…

Computer Vision and Pattern Recognition · Computer Science 2014-08-20 Eva Mohedano , Graham Healy , Kevin McGuinness , Xavier Giro-i-Nieto , Noel E. O'Connor , Alan F. Smeaton

Electron microscopy (EM) imaging offers unparalleled resolution for analyzing neural tissues, crucial for uncovering the intricacies of synaptic connections and neural processes fundamental to understanding behavioral mechanisms. Recently,…

Computer Vision and Pattern Recognition · Computer Science 2024-08-27 Ruohua Shi , Qiufan Pang , Lei Ma , Lingyu Duan , Tiejun Huang , Tingting Jiang