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Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…

Optics · Physics 2025-10-14 Guifeng Li , Chaoyang Gong

Single-pixel imaging, with the advantages of a wide spectrum, beyond-visual-field imaging, and robustness to light scattering, has attracted increasing attention in recent years. Fourier single-pixel imaging (FSI) can reconstruct sharp…

Image and Video Processing · Electrical Eng. & Systems 2021-08-05 Dong Zhou , Jie Cao , Huan Cui , Qun Hao , Bing-Kun Chen , Kai Lin

Hyperspectral imaging (HSI) enables detailed land cover classification, yet low spatial resolution and sparse annotations pose significant challenges. We present a label-efficient framework that leverages spatial features from a frozen…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yuzhen Hu , Biplab Banerjee , Saurabh Prasad

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Modulation-enhanced localization microscopy (MELM) has demonstrated significant improvements in both lateral and axial localization precision compared to conventional single-molecule localization microscopy (SMLM). However, lateral…

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Signal Processing · Electrical Eng. & Systems 2023-07-18 Olivier Leblanc , Matthias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

Optics · Physics 2024-11-18 Doron Shterman , Guy Bartal

Light field (LF) images acquired by hand-held devices usually suffer from low spatial resolution as the limited detector resolution has to be shared with the angular dimension. LF spatial super-resolution (SR) thus becomes an indispensable…

Image and Video Processing · Electrical Eng. & Systems 2021-10-11 Jing Jin , Junhui Hou , Zhiyu Zhu , Jie Chen , Sam Kwong

A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…

Optics · Physics 2010-08-27 Alexander R. Small

In the ELTs era, where the need for versatile and innovative solutions to produce very high spatial resolution images has become a major issue, the search of synergies with other science fields seems a logic step. One of the considered…

Instrumentation and Methods for Astrophysics · Physics 2018-11-15 E. Quevedo , S. Velasco , C. Colodro-Conde , G. Rodríguez-Coira , A. Oscoz , R. L. López , J. Font , J. H. Brito , O. Llinas , S. Robaina , G. M. Callico , R. Sarmiento , R. Rebolo

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel