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Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

To be able to resolve molecular-clusters it is crucial to access vital informations (such as, molecule density and cluster-size) that are key to understand disease progression and the underlying mechanism. Traditional single-molecule…

Quantitative Methods · Quantitative Biology 2021-01-27 Partha Pratim Mondal

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

State space models (SSMs) have recently achieved strong performance on long sequence modeling tasks while offering improved memory and computational efficiency compared to transformer based architectures. However, their evaluation has been…

Machine Learning · Computer Science 2026-03-13 Fatemeh Valeh , Monika Farsang , Radu Grosu , Gerhard Schütz

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

Super-resolution microscopy is providing unprecedented insights into biology by resolving details much below the diffraction limit. State-of-the-art Single Molecule Localization Microscopy (SMLM) techniques for super-resolution are…

Quantitative Methods · Quantitative Biology 2016-12-13 Krishna Agarwal , Radek Macháň

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by…

Quantitative Methods · Quantitative Biology 2023-08-09 Afonso Mendes , Hannah S. Heil , Simao Coelho , Christophe Leterrier , Ricardo Henriques

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…

Optics · Physics 2018-12-13 Oren Solomon , Yonina C. Eldar , Maor Mutzafi , Mordechai Segev

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Single-molecule localization microscopy (SMLM) surpasses the diffraction limit, achieving subcellular resolution. Traditional SMLM analysis methods often rely on point spread function (PSF) model fitting, limiting the application of complex…

Quantitative Methods · Quantitative Biology 2024-10-04 Tingdan Luo

Super-resolution is the process of obtaining a high-resolution image from one or more low-resolution images. Single image super-resolution (SISR) and multi-frame super-resolution (MFSR) methods have been evolved almost independently for…

Computer Vision and Pattern Recognition · Computer Science 2023-03-07 Mohammad Mahdi Afrasiabi , Reshad Hosseini , Aliazam Abbasfar

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by…

We report an experimental demonstration of a nonclassical imaging mechanism with super-resolving power beyond the Rayleigh limit. When the classical image is completely blurred out due to the use of a small imaging lens, by taking advantage…

Optics · Physics 2016-11-03 Jane Sprigg , Tao Peng , Yanhua Shih