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The study of transcription remains one of the centerpieces of modern biology with implications in settings from development to metabolism to evolution to disease. Precision measurements using a host of different techniques including…

Subcellular Processes · Quantitative Biology 2021-06-09 Muir J. Morrison , Manuel Razo-Mejia , Rob Phillips

Molecular chaperones are machines that consume copious amount of ATP to drive misfolded proteins or RNA to fold into functionally competent native states. Because the folding landscapes of biomolecules with complex native state topology are…

Biological Physics · Physics 2025-06-17 Changbong Hyeon , D. Thirumalai

A double stranded DNA molecule under the stress of a pulling force acting on the strand terminals exhibits a partially denatured structure or can be completely unzipped depending the magnitude of the pulling force. A scaling argument for…

Statistical Mechanics · Physics 2009-09-25 Jeff Z. Y. Chen

Gene transcription is a highly stochastic and dynamic process. As a result, the mRNA copy number of a given gene is heterogeneous both between cells and across time. We present a framework to model gene transcription in populations of cells…

Quantitative Methods · Quantitative Biology 2017-01-10 Justine Dattani , Mauricio Barahona

It is well known that transcription can induce torsional stress in DNA, affecting the activity of nearby genes or even inducing structural transitions in the DNA duplex. It has long been assumed that the generation of significant torsional…

Soft Condensed Matter · Physics 2009-10-31 Philip Nelson

Down regulation of mRNA translation is an important problem in various bio-medical domains ranging from developing effective medicines for tumors and for viral diseases to developing attenuated virus strains that can be used for…

Genomics · Quantitative Biology 2016-06-29 Yoram Zarai , Michael Margaliot , Tamir Tuller

Large-scale simultaneous mRNA translation and the resulting competition for the available ribosomes has important implications to the cell's functioning and evolution. Developing a better understanding of the intricate correlations between…

Genomics · Quantitative Biology 2015-08-12 Alon Raveh , Michael Margaliot , Eduardo D. Sontag , Tamir Tuller

In many organisms the expression levels of each gene are controlled by the activation levels of known "Transcription Factors" (TF). A problem of considerable interest is that of estimating the "Transcription Regulation Networks" (TRN)…

Applications · Statistics 2010-11-09 Gareth M. James , Chiara Sabatti , Nengfeng Zhou , Ji Zhu

DNA methylation is usually treated as an epigenetic memory mark: transcriptional history is written into regulatory DNA and later stabilizes a chosen cell identity. This picture explains persistence, but it makes memory passive. Here we…

Molecular Networks · Quantitative Biology 2026-05-15 Kaifeng Wang , Ming Han

We previously proposed the existence of DNA to DNA transcription in eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA) transcript is produced and released from the genome remains unknown. We once speculated that the…

Subcellular Processes · Quantitative Biology 2016-06-21 Gao-De Li

RNA polymerase (RNAP) elongates RNA by walking along a DNA template and selectively incorporating ribonucleoside triphosphates (rNTPs). Rather than mechanically replicating the base sequence, RNAP conditions binding and chemistry on the…

Biological Physics · Physics 2026-01-21 Tatsuaki Tsuruyama

MicroRNA-mediated regulation of gene expression is characterised by some distinctive features that set it apart from unregulated and transcription factor-regulated gene expression. Recently, a mathematical model has been proposed to…

Quantitative Methods · Quantitative Biology 2012-10-19 Indrani Bose , Sayantari Ghosh

The study of functional genomics--particularly in non-model organisms has been dramatically improved over the last few years by use of transcriptomes and RNAseq. While these studies are potentially extremely powerful, a computationally…

Genomics · Quantitative Biology 2013-07-25 Matthew D MacManes , Michael B Eisen

High-throughput transcriptomics experiments have reached the stage where the count of the number of reads alignable to a given position can be treated as an almost-continuous signal. This allows to ask questions of biophysical/biotechnical…

Genomics · Quantitative Biology 2014-10-02 Nicolas Innocenti , Erik Aurell

RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions. Yet, the underlying mechanisms and coding…

Biomolecules · Quantitative Biology 2007-05-23 A. Xayaphoummine , V. Viasnoff , S. Harlepp , H. Isambert

Systematic characterization of biological effects to genetic perturbation is essential to the application of molecular biology and biomedicine. However, the experimental exhaustion of genetic perturbations on the genome-wide scale is…

Genomics · Quantitative Biology 2024-03-06 Lingmin Zhan , Yuanyuan Zhang , Yingdong Wang , Aoyi Wang , Caiping Cheng , Jinzhong Zhao , Wuxia Zhang , Peng Lia , Jianxin Chen

Gene expression time delays, modelling the complex biological processes of gene transcription and translation, have been shown to play an important role in cellular dynamics. Time delays, motivated by the gene expression process, can also…

Cell Behavior · Quantitative Biology 2022-02-24 Alec Sargood

Many biological processes, from cell division to viral lysis, are triggered when an internal stochastic variable reaches a threshold. Here we introduce Branching under First-Passage Resetting, a general framework in which replication events…

Populations and Evolution · Quantitative Biology 2026-05-19 Aanjaneya Kumar , James Holehouse

Loop formation in long molecules occurs many places in nature, from solutions of carbon nanotubes to polymers inside a cell. In this article, we review theoretical studies of the static and dynamic properties of polymer loops. For example,…

Biological Physics · Physics 2007-05-23 Suckjoon Jun , John Bechhoefer

We demonstrate a catalytic control method for speeding up the single-molecule DNA motor introduced by Li and Tan [Nano Lett. {\bf 2}, 315 (2002)]. A key rate-limiting barrier in the reaction part of the cycle is the tendency for the second…

Soft Condensed Matter · Physics 2009-11-10 Yufang Wang , Y. Zhang , N. P. Ong
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