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Living cells provide a fluctuating, out-of-equilibrium environment in which genes must coordinate cellular function. DNA looping, which is a common means of regulating transcription, is very much a stochastic process; the loops arise from…

Biomolecules · Quantitative Biology 2015-05-18 Yih-Fan Chen , J. N. Milstein , Jens-Christian Meiners

Reconstructing transcriptional regulatory networks is an important task in functional genomics. Data obtained from experiments that perturb genes by knockouts or RNA interference contain useful information for addressing this reconstruction…

Machine Learning · Statistics 2015-06-18 Ali Shojaie , Alexandra Jauhiainen , Michael Kallitsis , George Michailidis

MicroRNAs can affect the protein translation using nine mechanistically different mechanisms, including repression of initiation and degradation of the transcript. There is a hot debate in the current literature about which mechanism and in…

Molecular Networks · Quantitative Biology 2013-04-09 A. Zinovyev , N. Morozova , A. N. Gorban , A. Harel-Belan

mRNA translation is a crucial process that leads to protein synthesis in living cells. Therefore, it is a process that needs to work optimally for a cell to stay healthy and alive. With advancements in microscopy and novel experimental…

Subcellular Processes · Quantitative Biology 2023-12-20 Binil Shyam T , Rati Sharma

The study of gene regulation and expression is often discussed in quantitative terms. In particular, the expression of genes is regularly characterized with respect to how much, how fast, when and where. Whether discussing the level of gene…

Molecular Networks · Quantitative Biology 2007-05-23 Lacramioara Bintu , Nicolas E. Buchler , Hernan G. Garcia , Ulrich Gerland , Terence Hwa , Jane' Kondev , Rob Phillips

Both multi-cell biochemical assays and single cell fluorescence measurements have revealed that the elongation rate of Polymerase II (PolII) in eukaryotes varies largely across different cell types and genes. However, there is not yet a…

Subcellular Processes · Quantitative Biology 2015-04-23 Paolo Annibale , Enrico Gratton

Large scale surveys in mammalian tissue culture cells suggest that the protein expressed at the median abundance is present at 8,000 - 16,000 molecules per cell and that differences in mRNA expression between genes explain only 10-40% of…

Genomics · Quantitative Biology 2021-12-01 Jingyi Jessica Li , Peter J. Bickel , Mark D. Biggin

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik

It has been suggested by several authors that nonlinear excitations, in particular solitary waves, could play a fundamental functional role in the process of DNA transcription, effecting the opening of the double chain needed for RNA…

Other Quantitative Biology · Quantitative Biology 2016-09-08 G. Gaeta

Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as…

Biological Physics · Physics 2011-01-28 Ajeet K. Sharma , Debashish Chowdhury

We investigate delay effects on dominant transition pathways (DTP) between metastable states of stochastic systems. A modified version of the Maier-Stein model with linear delayed feedback is considered as an example. By a stability…

Applications · Statistics 2012-09-19 Huijun Jiang , Zhonghuai Hou

microRNAs (miRNAs) regulate gene expression at post-transcriptional level by repressing target RNA molecules. Competition to bind miRNAs tends in turn to correlate their targets, establishing effective RNA-RNA interactions that can…

Molecular Networks · Quantitative Biology 2020-07-01 Mattia Miotto , Enzo Marinari , Andrea De Martino

Many {\it ribosomes} simultaneously move on the same messenger RNA (mRNA), each synthesizing separately a copy of the same protein. In contrast to the earlier models, here {\it we develop a ``unified'' theoretical model} that not only…

Biological Physics · Physics 2007-05-23 Aakash Basu , Debashish Chowdhury

DNA and RNA are generally regarded as central molecules in molecular biology. Recent advancements in the field of DNA/RNA nanotechnology successfully used DNA/RNA as programmable molecules to construct molecular machines and nanostructures…

Biomolecules · Quantitative Biology 2019-04-01 Fan Hong , Petr Šulc

Collective coordinate and direct numerical integration methods are applied to the analysis of a one-dimensional DNA model. A modification of the coupling constant in an extended region is found to be less selective towards the breather it…

Condensed Matter · Physics 2009-10-28 Julian J. -L. Ting , Michel Peyrard

We introduce a two-species exclusion model to describe the key features of the conflict between the RNA polymerase (RNAP) motor traffic, engaged in the transcription of a segment of DNA, concomitant with the progress of two DNA replication…

Subcellular Processes · Quantitative Biology 2018-04-18 Soumendu Ghosh , Bhavya Mishra , Shubhadeep Patra , Andreas Schadschneider , Debashish Chowdhury

Chaperone-assisted translocation through a nanopore embedded in membrane holds a prominent role in the transport of biopolymers. Inspired by classical Brownian ratchet, we develop a theoretical framework characterizing such translocation…

Biological Physics · Physics 2020-02-17 Xining Xu , Yunxin Zhang

Solid-state nanopore DNA sequencers present mechanical and chemical stability, reusability, and large-scale integrability. However, their development is hindered by the absence of a protein-free mechanism for controlling DNA translocation,…

Biological Physics · Physics 2026-03-17 Tomoki Ohkubo

The dynamics of the DNA denaturation is studied using the Peyrard-Bishop-Dauxois model. The denaturation rate of double stranded polymers decreases exponentially as function of length below the denaturation temperature. Above Tc, the rate…

Biological Physics · Physics 2015-06-04 Titus S. van Erp , Michel Peyrard

Recently, several studies have investigated the transcription process associated to specific genetic regulatory networks. In this work, we present a stochastic approach for analyzing the dynamics and effect of negative feedback loops (FBL)…

Molecular Networks · Quantitative Biology 2007-08-20 J. C. Nacher , T. Ochiai