Related papers: Holographic laser Doppler imaging of pulsatile blo…
We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency…
We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an…
Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry…
We report a pilot study with a wide-field laser Doppler detection scheme used to perform laser Doppler anemometry and imaging of particle seeded microflow. The optical field carrying the local scatterers (particles) dynamic state, as a…
We performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate…
Laser Doppler holography was introduced as a full-field imaging technique to measure blood flow in the retina and choroid with an as yet unrivaled temporal resolution. We here investigate separating the different contributions to the power…
A comprehensive assessment of retinal health demands reliable and precise methods to measure localized blood perfusion. Despite considerable advancements in imaging techniques, such as indocyanine green and fluorescein angiography, along…
Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making…
We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and…
Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show…
Full-field swept-source optical coherence tomography (FF-SS-OCT) and laser Doppler holography (LDH) are two holographic imaging techniques presenting unique capabilities for ophthalmology. We report on interlaced FF-SS-OCT and LDH imaging…
We report on an experimental demonstration of surface acoustic waves monitoring on a thin metal plate with heterodyne optical holography. Narrowband imaging of local optical pathlength modulation is achieved with a frequency-tunable…
The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome…
We report on using a laser Doppler processing of Fourier-domain optical coherence tomography (OCT) data for the assessment of pulsatile blood flow in the choriocapillaris. Signal fluctuations in B-scans recorded at 2 kHz were analyzed by…
We report laser Doppler ophthalmoscopic fundus imaging in the rat eye with near-IR heterodyne holography. Sequential sampling of the beat of the reflected radiation against a frequency-shifted optical local oscillator is made onto an array…
High-speed digital holography reveals local blood flow contrasts in the eye fundus through deterministic signal analysis, leveraging a forward scattering model of dynamically diffused light. This approach enables the estimation of absolute…
The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure…
Laser Doppler holography (LDH) is a full-field interferometric imaging technique recently applied in ophthalmology to measure blood flow, a parameter of high clinical interest. From the temporal fluctuations of digital holograms acquired at…
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…
This study presents a novel quantitative estimation method for total retinal arterial blood flow utilizing real-time Doppler holography at an unprecedented frame rate of 33,000 frames per second. This technique, leveraging high-speed…