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Total Internal Reflection Microscopy (TIRM) is a sensitive non-invasive technique to measure the interaction potentials between a colloidal particle and a wall with femtonewton resolution. The equilibrium distribution of the particle-wall…

Total internal reflection microscopy (TIRM) measures changes in the distance between a colloidal particle and a transparent substrate by measuring the intensity of light scattered by the particle when it is illuminated by the evanescent…

Soft Condensed Matter · Physics 2021-11-25 Fan Cui , David J. Pine

Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to…

Optics · Physics 2014-06-30 R S Dutra , N B Viana , P A Maia Neto , H M Nussenzveig

We explain and demonstrate a new method of force- and position-calibration for optical tweezers with back-focal-plane photo detection. The method combines power spectral measurements of thermal motion and the response to a sinusoidal motion…

Optical Deflectometric Tomography (ODT) provides an accurate characterization of transparent materials whose complex surfaces present a real challenge for manufacture and control. In ODT, the refractive index map (RIM) of a transparent…

Computer Vision and Pattern Recognition · Computer Science 2013-10-31 Adriana Gonzalez , Laurent Jacques , Christophe De Vleeschouwer , Philippe Antoine

Optical tweezers (OT) have become an essential technique in several fields of physics, chemistry, and biology as precise micromanipulation tools and microscopic force transducers. Quantitative measurements require the accurate calibration…

In recent years, levitated particles of optical traps in vacuum have shown enormous potential in precision sensor development and searching for new physics. The accuracy of the calibration relating the detected signal to absolute…

Instrumentation and Detectors · Physics 2022-03-08 Jianyu Yang , Nan Li , Xunmin Zhu , Ming Chen , Xingfan Chen , Cheng Liu , Jian Zhuang , Huizhu Hu

We describe a method that uses total internal reflection at the water-air interface inside a large, transparent tank, to measure the interface's deflections. Using this configuration, we obtain an optical set-up where the liquid surface…

Fluid Dynamics · Physics 2021-10-26 Utkarsh Jain , Anaïs Gauthier , Devaraj van der Meer

Dielectric tensor tomography is an imaging technique for mapping three-dimensional distributions of dielectric properties in transparent materials. This work introduces an enhanced illumination strategy employing a micro-electromechanical…

Optical tweezers equipped with position detection allow for application of piconewton-scale forces and high-temporal-resolution measurements of nanometer-scale motion. While typically used for trapping microscopic objects, the optical…

For measurements designed to accurately determine layer thickness, there is a natural trade-off between sensitivity to optical thickness and lateral resolution due to the angular ray distribution required for a focused beam. We demonstrate…

Total internal reflection (TIR) is a ubiquitous phenomenon used in photonic devices ranging from waveguides and resonators to lasers and optical sensors. Controlling this phenomenon and light confinement are keys to the future integration…

Optics · Physics 2015-11-17 Saman Jahani , Zubin Jacob

Multi-dielectric coated coverslip can be designed to reach large optical field enhancements when working under proper illumination conditions and in total internal reflection. In an objective-based total internal reflection fluorescence…

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim

A standard paradigm of localization microscopy involves extension from two to three dimensions by engineering information into emitter images, and approximation of errors resulting from the field dependence of optical aberrations. We invert…

Accurate and high-efficiency film metrology remains a key challenge in High-Volume Manufacturing (HVM), where conventional spectroscopic reflectometry and white light interferometry (WLI) are either limited by model dependence or…

Optical and acoustical trapping has been established as a tool for holding and moving microscopic particles suspended in a liquid in a contact-free and non-invasive manner. Opposed to standard microscopic imaging where the probe is fixated,…

Numerical Analysis · Mathematics 2025-10-27 Peter Elbau , Monika Ritsch-Marte , Otmar Scherzer , Denise Schmutz

The optics of a number of future X-ray telescopes will have very long focal lengths (10 - 20 m), and will consist of a number of nested/stacked thin, grazing-incidence mirrors. The optical quality characterization of a real mirror can be…

Instrumentation and Methods for Astrophysics · Physics 2015-09-09 D. Spiga , S. Basso , M. Bavdaz , V. Burwitz , M. Civitani , O. Citterio , M. Ghigo , G. Hartner , B. Menz , G. Pareschi , L. Proserpio , B. Salmaso , G. Tagliaferri , E. Wille

Sub-micrometer scale light patterns play a pivotal role in various fields, including biology, biophysics, and AMO physics. High-resolution, in situ observation of light profiles is essential for their design and application. However,…

Optical tweezers can manipulate tiny particles. However, the distortion caused by the scattering medium restricts the applications of optical tweezers. Wavefront shaping techniques including the transmission matrix (TM) method are powerful…

Optics · Physics 2022-01-17 Kaige Liu , Hengkang Zhang , Shanshan Du , Zeqi Liu , Bin Zhang , Xing Fu , Qiang Liu
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