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Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

Many applications seek to measure a sample's absorption coefficient spectrum to retrieve the chemical makeup. Many real world samples are optically turbid, causing scattering confounds which many commercial spectrometers cannot address.…

Optics · Physics 2023-01-05 Giles Blaney , Angelo Sassaroli , Sergio Fantini

We present a computational method for full-range interferometric synthetic aperture microscopy (ISAM) under dispersion encoding. With this, one can effectively double the depth range of optical coherence tomography (OCT), whilst…

Image and Video Processing · Electrical Eng. & Systems 2020-02-03 Jonathan H. Mason , Mike E. Davies , Pierre O. Bagnaninchi

Aiming at absolute force calibration of optical tweezers, following a critical review of proposed theoretical models, we present and test the results of MDSA (Mie-Debye-Spherical Aberration) theory, an extension of a previous (MD) model,…

Optical tweezers find applications in various fields, ranging from biology to physics. One of the fundamental steps necessary to perform quantitative measurements using trapped particles is the calibration of the tweezer's spring constant.…

Quantum Physics · Physics 2020-06-05 Bruno Melo , Felipe Almeida , Guilherme Temporão , Thiago Guerreiro

We present an experimental technique that enables the preparation of defect-free arrays of 87Rb atoms within a microscopic high-finesse optical standing-wave cavity. By employing optical tweezers, we demonstrate atom positioning with a…

Quantum Physics · Physics 2025-02-20 Matthias Seubert , Lukas Hartung , Stephan Welte , Gerhard Rempe , Emanuele Distante

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

Optics · Physics 2024-11-18 Doron Shterman , Guy Bartal

We report for the first time the theory of optical tweezers of spherical dielectric particles embedded in a chiral medium. We develop a partial-wave (Mie) expansion to calculate the optical force acting on a dielectric microsphere…

Optics · Physics 2020-09-28 Rfaqat Ali , R. S. Dutra , F. A. Pinheiro , F. S. S. Rosa , P. A. Maia Neto

Transient Photoluminescence Microscopy (TPLM) allows for the direct visualization of carrier transport in semiconductor materials with sub nanosecond and few nanometer resolution. The technique is based on measuring changes in the spatial…

We have addressed the challenge to carry out the angular tilt stabilization of a laser guiding mirror which is intended to route a laser beam with a high energy density. Such an application requires good angular accuracy as well as large…

Instrumentation and Detectors · Physics 2022-10-25 Martin Šarbort , Šimon Řeřucha , Petr Jedlička , Josef Lazar , Ondrej Číp

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

A methodology is introduced that enables an absolute, quantum-limited measurement of sub-wavelength interferometric displacements. The technique utilizes a high-frequency optical path modulation within an interferometer operated in a…

Quantum Physics · Physics 2016-02-12 Valérian Thiel , Pu Jian , Claude Fabre , Nicolas Treps , Jonathan Roslund

Various optical methods for measuring positions of micro-objects in 3D have been reported in the literature. Nevertheless, majority of them are not suitable for real-time operation, which is needed, for example, for feedback position…

Instrumentation and Detectors · Physics 2017-02-02 Martin Gurtner , Jiří Zemánek

Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

We introduce a new method, rooted in estimation theory, to detect individual atoms in site-resolved images of microtrap arrays, such as optical lattices or optical tweezers arrays. Using labelled test images, we demonstrate drastic…

We present a novel method for extrinsically calibrating a camera and a 2D Laser Rangefinder (LRF) whose beams are invisible from the camera image. We show that point-to-plane constraints from a single observation of a V-shaped calibration…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Wenbo Dong , Volkan Isler

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and…

Biomolecules · Quantitative Biology 2015-06-24 Zhaokun Zhou , Helen Miller , Adam J. M. Wollman , Mark C. Leake

The absolute reflectance of a reflector and its point spread function are the key parameters of a telescope for measuring light flux. Typically, one is using low-cost technologies for producing mirrors for the needs of astro-particle…

Instrumentation and Methods for Astrophysics · Physics 2018-10-22 Razmik Mirzoyan , Cornelia Arcaro , Hanna Kellermann , Markus Garczarczyik

Optical tweezers constitute pivotal tools in Atomic, Molecular, and Optical(AMO) physics, facilitating precise trapping and manipulation of individual atoms and molecules. This process affords the capability to generate desired geometries…

Atomic Physics · Physics 2024-01-11 Yongwoong Lee , Eunmi Chae

We demonstrate number-resolved detection of individual strontium atoms in a long working distance low numerical aperture (NA = 0.26) tweezer. Using a camera based on single-photon counting technology, we determine the presence of an atom in…

Atomic Physics · Physics 2020-03-11 N. C. Jackson , R. K. Hanley , M. Hill , F. Leroux , C. S. Adams , M. P. A. Jones