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Biological cells replicate their genomes in a well-planned manner. The DNA replication program of an organism determines the timing at which different genomic regions are replicated, with fundamental consequences for cell homeostasis and…

Subcellular Processes · Quantitative Biology 2024-05-28 Florian Pflug , Deepak Bhat , Simone Pigolotti

Genome replication, a key process for a cell, relies on stochastic initiation by replication origins, causing a variability of replication timing from cell to cell. While stochastic models of eukaryotic replication are widely available, the…

Genomics · Quantitative Biology 2017-08-23 Qing Zhang , Federico Bassetti , Marco Gherardi , Marco Cosentino Lagomarsino

In eukaryotes, DNA replication is initiated along each chromosome at multiple sites called replication origins. Locally, each replication origin is "licensed", or specified, at the end of the M and the beginning of G1 phases of the cell…

Genomics · Quantitative Biology 2008-01-21 John Herrick , Aaron Bensimon

Eukaryotic cells are often exposed to fluctuations in growth conditions as well as endogenous and exogenous stress-related agents. In addition, during development global patterns of gene transcription change dramatically, and these changes…

Genomics · Quantitative Biology 2008-12-23 John Herrick

The metazoan genome is replicated in precise cell lineage specific temporal order. However, the mechanism controlling this orchestrated process is poorly understood as no molecular mechanisms have been identified that actively regulate the…

Subcellular Processes · Quantitative Biology 2014-03-12 Yevgeniy Gindin , Manuel S. Valenzuela , Mirit I. Aladjem , Paul S. Meltzer , Sven Bilke

Transcription regulation is largely governed by the profile and the dynamics of transcription factors' binding to DNA. Stochastic effects are intrinsic to this dynamics and the binding to functional sites must be controled with a certain…

Genomics · Quantitative Biology 2007-05-23 Erik Aurell , Aymeric Fouquier d'Herouel , Claes Malmnas , Massimo Vergassola

A correlation between karyotype diversity and species richness was first observed in mammals in 1980, and subsequently confirmed after controlling for phylogenetic signal. The correlation was attributed to submicroscopic factors, presumably…

Populations and Evolution · Quantitative Biology 2025-11-18 John Herrick

We formulate a kinetic model of DNA replication that quantitatively describes recent results on DNA replication in the in vitro system of Xenopus laevis prior to the mid-blastula transition. The model describes well a large amount of…

Biological Physics · Physics 2018-11-06 John Herrick , Suckjoon Jun , John Bechhoefer , Aaron Bensimon

Recent genome-wide measurements of binding preferences of ~200 transcription regulators in the vicinity of transcription start sites in yeast, have provided a unique insight into the cis- regulatory code of a eukaryotic genome (Venters et…

Biomolecules · Quantitative Biology 2012-04-19 Ariel Afek , David B. Lukatsky

The dynamics of gene transcription is tightly regulated in eukaryotes. Recent experiments have revealed various kinds of transcriptional dynamics, such as RNA polymerase II pausing, that involves regulation at the transcription initiation…

Cell Behavior · Quantitative Biology 2015-08-11 Yucheng Hu , John Lowengrub

Till now, in biological sciences, the term, transcription, mainly refers to DNA to RNA transcription. But our recently published experimental findings obtained from Plasmodium falciparum strongly suggest the existence of DNA to DNA…

Subcellular Processes · Quantitative Biology 2018-01-30 Gao-De Li

Replication of genetic material is an important process for all living organisms. Origins of replication initiate the copying of DNA at many points on a chromosome, and it is the distribution of these points that is relevant here, as it…

Quantitative Methods · Quantitative Biology 2025-10-16 Huw Day , N C Snaith

Transcription factor proteins bind specific DNA sequences to control the expression of genes. They contain DNA binding domains which belong to several super-families, each with a specific mechanism of DNA binding. The total number of…

Biomolecules · Quantitative Biology 2010-07-27 Shalev Itzkovitz , Tsvi Tlusty , Uri Alon

Background: In Escherichia coli, overlapping rounds of DNA replication allow the bacteria to double in faster times than the time required to copy the genome. The precise timing of initiation of DNA replication is determined by a regulatory…

Many organisms possess both a cell cycle to control DNA replication and a circadian clock to anticipate changes between day and night. In some cases, these two rhythmic systems are known to be coupled by specific, cross-regulatory…

Molecular Networks · Quantitative Biology 2016-04-12 Joris Paijmans , Mark Bosman , Pieter Rein ten Wolde , David K. Lubensky

We present a comparative analysis of large-scale topological and evolutionary properties of transcription networks in three species, the two distant bacteria E. coli and B. subtilis, and the yeast S. cerevisiae. The study focuses on the…

Molecular Networks · Quantitative Biology 2008-05-16 A. L. Sellerio , B. Bassetti , H. Isambert , M. Cosentino Lagomarsino

Models of transcriptional regulation that assume equilibrium binding of transcription factors have been very successful at predicting gene expression from sequence in bacteria. However, analogous equilibrium models do not perform as well in…

Molecular Networks · Quantitative Biology 2021-10-14 Benjamin Zoller , Thomas Gregor , Gašper Tkačik

DNA replication is an essential process in biology and its timing must be robust so that cells can divide properly. Random fluctuations in the formation of replication starting points, called origins, and the subsequent activation of…

Quantitative Methods · Quantitative Biology 2012-02-03 Jens Karschau , J. Julian Blow , Alessandro P. S. de Moura

We previously proposed the existence of DNA to DNA transcription in eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA) transcript is produced and released from the genome remains unknown. We once speculated that the…

Subcellular Processes · Quantitative Biology 2016-06-21 Gao-De Li

Asynchronous replication of the genome has been associated with different rates of point mutation and copy number variation (CNV) in human populations. Here, we explored if the bias in the generation of CNV that is associated to DNA…

Genomics · Quantitative Biology 2013-11-27 David Juan , Daniel Rico , Tomas Marques-Bonet , Oscar Fernandez-Capetillo , Alfonso Valencia
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