Related papers: OpenSPIM - an open access platform for light sheet…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…
The dynamic architecture of the microtubule cytoskeleton is crucial for cell division, motility and morphogenesis. The dynamic properties of microtubules - growth, shrinkage, nucleation and severing - are regulated by an arsenal of…
MuSim is a new user-friendly program designed to interface to many different particle simulation codes, regardless of their data formats or geometry descriptions. It presents the user with a compelling graphical user interface that includes…
Background: Increasingly, decision-making in healthcare relies on computer models, be it clinical prediction models at point of care or decision-analytic models at the policymaking level. Given the important role models play in both…
Automated and computerised control of scientific instrumentation is almost ubiquitous in the modern laboratory. Most instrumentation is controlled over decades old communication busses or is accessed via proprietary system libraries. This…
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…
In this paper, we provide assembly instructions for an easy to build experimental setup in order to gain practical experience with tomography. In view of this, this paper can be seen as a complementary work to excellent mathematical…
High resolution light-sheet optical microscopy requires illuminating a sample by a thin excitation light sheet with large area. Here, we describe methods for producing light sheets with extended length, as compared to Gaussian-beam-type…
QuPath is open-source software for bioimage analysis. As a desktop application that is flexible and easy to install, QuPath is used by labs worldwide to visualise and analyse large and complex images. However, relying only on images stored…
We report the realisation of a high-finesse open-access cavity array, tailored towards the creation of multiple coherent light-matter interfaces within a compact environment. We describe the key technical developments put in place to…
Silicon Photo-Multipliers (SiPM) are state of the art light detectors with unprecedented single photon sensitivity and photon number resolving capability, representing a breakthrough in several fundamental and applied Science domains. An…
We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…
Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to a quantitative tool with ever-increasing resolution and throughput. Artificial intelligence, deep…
We report on an educational pilot program for low-cost physics experimentation run in Ecuador, South Africa, and the United States. The program was developed after having needs-based discussions with African educators, researchers, and…
Leaky radiation microscopy (LRM) is used to directly image the surface plasmon-coupled emission (SPCE). When compared with the prism based set-up commonly used in SPCE research, LRM has the advantages of directly giving out the emitting…
Optically-transparent opportunistic electromagnetic skins (OTO-EMSs) are proposed to enable outdoor-to-indoor (O2I) millimiter-wave (mmW) wireless communications with existing windows/glass-panels. More in detail, static passive EMSs…
Endoscopic optical coherence tomography (OCT) is a valuable tool for providing diagnostic images of internal organs and guiding interventions in real time. Miniaturized OCT endoscopes are essential for imaging small and convoluted luminal…
An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…
A reformulated implementation of single-sideband ptychography enables analysis and display of live detector data streams in 4D scanning transmission electron microscopy (STEM) using the LiberTEM open-source platform. This is combined with…